Year 2018

• Modification of quaternary structure of Candida albicans GlcN-6-P synthase and its desensitization to inhibition by UDP-GlcNAc by site-directed mutagenesis

  Publication

  • K. Kwiatkowska-Semrau
  • M. Wojciechowski
  • I. Gabriel
  • S. Crucho
  • S. Milewski

  - BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS - Year 2018

  Site-directed mutagenesis of the CaGFA1 gene encoding glucosamine-6-phosphate synthase from Candida albicans was performed. Desensitization of the enzyme to inhibition by UDPGlcNAc was achieved upon T487I and H492F substitutions at the UDP-GlcNAc binding site, exchange of D524, S525 and S527 for Ala at the dimer:dimer interface and construction of the tail-lock array (L434R and L460A) at the C-tail region. The first two sets if...

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Year 2015

• Heterogeneity of quaternary structure of glucosamine-6-phosphate deaminase from Giardia lamblia

  Publication

  • K. Kwiatkowska-Semrau
  • J. Czarnecka
  • M. Wojciechowski
  • S. Milewski

  - PARASITOLOGY RESEARCH - Year 2015

  The oligoHis-tagged versions of glucosamine-6- phosphate deaminase from Giardia lamblia (GlmNagB-HisN, GlmNagB-HisC) were constructed and purified to hear homogeneity, and their kinetic and structural properties were compared to those of the wild-type enzyme (GlmNagB). Introduction of the oligoHis tag at the GlmNagB C-terminus resulted in almost complete loss of the catalytic activity, while the catalytic properties of GlmNagB-HisN...

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Year 2013

• Desensitization of glucosamine-6-phosphate synthase to inhibition by UDP-GlcNAc

  Publication

  • K. Kwiatkowska-Semrau
  • S. Milewski
  • A. Napiórkowska

  - Acta Biochimica Polonica - Year 2013

  Glucosamine-6-phosphate (GlcN-6-P) synthase, known also as L-Glutamine: D-fructose-6-phosphate amidotransferase, catalyzes the first committed step in the pathway leading to the ultimate formation of UDP-GlcNAc. The final product of this pathway is an activated precursor of numerous macromolecules containing amino sugars, including chitin and mannproteins in fungi, peptidoglycan and lipopolysaccharides in bacteria, and glycoproteins...

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• Synthesis of phosphono and phosphate derivatives of hydroxyimino-D-alditols as new potential antifungal agents

  Publication

  • M. Cyman
  • H. Myszka
  • K. Kwiatkowska-Semrau
  • S. Milewski
  • B. Liberek

  - GLYCOCONJUGATE JOURNAL - Year 2013

  In search for new effective we antyfungals we focus on two enzymes involved in biosynthesis of the fungal cell wall. The first enzyme is glucosamine-6-phosphate (GlmS), which catalyzes transformation of D-fructose-6-phosphate (Fru-6P) to D-glucosamine-6-phodphate (GlcN-6P) in the chitin biosynthesis pathway. The second enzyme is phosphomannose isomerase (PMI) repored to play a crucial role in biosynthesis of many mannosylated structures,...

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