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Description
This dataset contains experimental results from electrochemical studies of aptamer-based biosensors composed of self-organised AuNC at GC electrode for the rapid detection of E. coli in real human urine samples through polymerase RNA. The study discusses the electrode in each functionalisation step by attaching a non-coding strand of the oligonucleotide and attaching the complementary strand in the hybridisation process. The dataset includes functionalisation steps that lead to an increase in the thickness of the layer functionalised at the electrode surface, which successively increases the charge transfer resistance, RCT and differences in the charge transfer kinetics process. All electrochemical measurements were performed on an Autolab M204 potentiostat (Metrohm) using a three-electrode system. CV and EIS measurements were conducted in a solution consisting of equimolar amounts of 1 mM K3[Fe(CN)6 ] and 1mM [Fe(CN)6] dissolved in 0.01 M PBS (pH 7.4). The impedance monitoring (DEIS) was performed using an AC signal consisting of a package of 21 elementary sinusoids, ranging from 3 to 4500 Hz, superimposed on a DC voltammetry scan, from − 0.35 V to 0.60 V, scan rate 2 mV/s. The DEIS measurements were used to perform the multiparametric impedance discriminant analysis (MIDA).
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
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CC BYAttribution - Raw data:
- Data contained in dataset was not processed.
Details
- Year of publication:
- 2023
- Verification date:
- 2025-03-17
- Dataset language:
- English
- Fields of science:
-
- materials engineering (Engineering and Technology)
- DOI:
- DOI ID 10.34808/cw4t-3q66 open in new tab
- Funding:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset Electrochemical studies of AuNC on GC, ITO and Au transducers
- publication Deciphering the Molecular Mechanism of Substrate-Induced Assembly of Gold Nanocube Arrays toward an Accelerated Electrocatalytic Effect Employing Heterogeneous Diffusion Field Confinement
- dataset Scanning Electron Microscopy images of nanocubes suspension 1 month after synthesis
- publication Discriminating macromolecular interactions based on an impedimetric fingerprint supported by multivariate data analysis for rapid and label-free Escherichia coli recognition in human urine
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