Application of the algal model to determine the non-steroidal anti-inflammatory drugs mitotoxicity – diclofenac as a case study - Project - Bridge of Knowledge

Your account

login

Search

Application of the algal model to determine the non-steroidal anti-inflammatory drugs mitotoxicity – diclofenac as a case study

Diclofenac (DF), belonging to the non-steroidal anti-inflammatory drugs (NSAIDs), is one of the pharmaceuticals on the list of priority hazardous substances for environment. Pharmaceuticals, designed to have a specific therapeutic effect on humans and animals, could have significant toxic effect on non-target organisms living in polluted areas. It was shown that in animal cells DF disrupt mitochondrion functioning due to uncoupling of oxidative phosphorylation by the opening of membrane permeability transition pore in the mitochondria, which leads to the loss of the transmembrane potential required for synthesis of ATP. DF also disturb Ca2+ homeostasis because it blocks NMDA-receptor, the glutamate-gated channel responsible for Ca2+ transport to the cell. Analogs of the above channels were found in plants and algae which suggests that DF would affect them in a similar way. Because Ca2+ is necessary to keep mitochondrion and chloroplast (energetic centers of plant cells) operate, it cannot be excluded that changes in intracellular Ca2+concentration, leading to disruption of cell bioenergetics, could be one of the reasons of DF phytotoxicity. In few reported studies concerning pharmaceuticals effect on plants, photosynthesis disruption was shown but respiratory processes were not investigated in detail. Thus, the aim of the project is to verify the hypothesis that one of the causes of DF phytotoxicity is the disorder of plant mitochondrion functioning, based on the model green algae Chlamydomonas reinhardtii. 

Details

Project's acronym:
AMDDM: Algal Model for the Determination of Diclofenac Mitotoxicity
Financial Program Name:
PRELUDIUM
Organization:
Narodowe Centrum Nauki (NCN) (National Science Centre)
Agreement:
2021/41/N/NZ8/00124 z dnia 2022-01-13
Realisation period:
2022-01-13 - 2025-01-12
Research team leader:
Darya Harshkova
Team members:
Realised in:
Uniwersytet Gdanski
Project's value:
209 440.00 PLN
Request type:
National Research Programmes
Domestic:
Domestic project
Verified by:
No verification

Referenced datasets

seen 106 times