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A novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with Candida albicans
Abstract
Aims: This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. Methods and Results: The effect of Candida infection on host cell viability was evaluated by the microscopy of trypan blue-stained cells and lactate dehydrogenase (LDH) activity. The in vitro ‘drug potency assay’ utilized the Cell Counting Kit-8 and measured post-antifungal treatment viability of Candida-infected HL-60 cells and the ability of the antifungal treatment to prevent infection. LDH activity showed that 42% 40 and 853% 740 of HL-60 cells were killed following Candida infection at the multiplicity of infection (MOI) of 1 : 1 and 1 : 5, respectively. The antifungal nystatin (078–25 lmol l 1) was found to inhibit C. albicans infection as seen by the significantly increased viability of HL-60 cells. Cytotoxicity of nystatin towards infected HL-60 cells was evident at higher concentrations and this was also confirmed by propidium iodide staining. Conclusions: An assay using undisturbed cell suspension conditions was successfully developed for assessing the selectivity of the antifungal therapy in the host-Candida environment. Significance and Impact of the Study: The assay employing Candida infection of host cell suspensions represents a promising method for testing interactions of antifungal compounds with both fungal and host cells.
Citations
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Authors (5)
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Ł Turczyk
- University of Gdańsk Department of Molecular Enzymology
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X. Wei
- Cardiff University .
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David W. Williams
- Cardiff University .
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF APPLIED MICROBIOLOGY
no. 119,
edition 1,
pages 177 - 187,
ISSN: 1364-5072 - Language:
- English
- Publication year:
- 2015
- Bibliographic description:
- Boros-Majewska J., Turczyk Ł., Wei X., Milewski S., Williams D. W.: A novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with Candida albicans// JOURNAL OF APPLIED MICROBIOLOGY. -Vol. 119, iss. 1 (2015), s.177-187
- DOI:
- Digital Object Identifier (open in new tab) 10.1111/jam.12817
- Verified by:
- Gdańsk University of Technology
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