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Abstract
Studies of 54 antioxidants revealed that 27 of them, mainly polyphenols, generated hydrogen peroxide (H2O2) when added to Dulbecco’s modified Eagle’s medium (DMEM), other media used for culture of mammalian and yeast cells and phosphate-buffered saline. The most active antioxidants were: propyl gallate (PG), (−)-epigallocatechin gallate (EGCG) and quercetin (Q). Chelex treatment and iron chelators decreased H2O2 generation suggesting that transition metal ions catalyze antioxidant autoxidation and H2O2 production. Green tea also generated H2O2; tea prepared on tap water generated significantly more H2O2 than tea prepared on deionized water. Ascorbic acid decreased H2O2 production although it generated H2O2 itself, in the absence of other additives. Lemon added to the tea significantly reduced generation of H2O2. Hydrogen peroxide generated in the medium contributed to the cytotoxicity of PG, EGCG and Q to human prostate carcinoma DU-145 cells, since catalase increased the survival of the cells subjected to these compounds in vitro.
Authors (6)
-
Michalina Grzesik mgr inż.
- Uniwersytet Rzeszowski/Wydział Biologiczno - Rolniczy Zakład Biochemii Analitycznej
-
Grzegorz Bartosz Prof. dr hab.
- Uniwersytet Łódzki/Wydział Biologii i Ochrony Środowiska KATEDRA BIOFIZYKI MOLEKULARNEJ
-
Ireneusz Stefaniuk Dr
- Uniwersytet Rzeszowski Cetrum Dydaktyczno Naukowe Mikroelektroniki i Nanotechnologii
-
Monika Pichla
- Uniwersytet Rzeszowski/Wydział Biologiczno - Rolniczy Zakład Biochemii Analitycznej
-
Izabela Sadowska-Bartosz
- Uniwersytet Rzeszowski/Wydział Biologiczno - Rolniczy Zakład Biochemii Analitycznej
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
FOOD CHEMISTRY
no. 278,
pages 692 - 699,
ISSN: 0308-8146 - Language:
- English
- Publication year:
- 2019
- Bibliographic description:
- Grzesik M., Bartosz, G., Stefaniuk, I., Pichla M., Namieśnik J., Sadowska-Bartosz I.: Dietary antioxidants as a source of hydrogen peroxide// FOOD CHEMISTRY. -Vol. 278, (2019), s.692-699
- Verified by:
- Gdańsk University of Technology
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