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Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots
Abstract
Microshoot cultures of the North American endemic Salvia apiana were established for the first time and evaluated for essential oil production. Stationary cultures, grown on Schenk-Hildebrandt (SH) medium, supplemented with 0.22 mg/L thidiazuron (TDZ), 2.0 mg/L 6-benzylaminopurine and 3.0% (w/v) sucrose, accumulated 1.27% (v/m dry weight) essential oil, consisting mostly of 1,8-cineole, β-pinene, α-pinene, β-myrcene and camphor. The microshoots were adapted to agitated culture, showing biomass yields up to ca. 19 g/L. Scale-up studies demonstrated that S. spiana microshoots grow well in temporary immersion systems (TIS). In the RITA bioreactor, up to 19.27 g/L dry biomass was obtained, containing 1.1% oil with up to ca. 42% cineole content. The other systems employed, i.e. Plantform (TIS) and a custom made spray bioreactor (SGB), yielded ca. 18 and 19 g/L dry weight, respectively. The essential oil content of Plantform and SGB-grown microshoots was comparable to RITA bioreactor, however, the content of cineole was substantially higher (ca. 55%). Oil samples isolated from in vitro material proved to be active in acetylcholinesterase (up to 60.0% inhibition recorded for Plantform-grown microshoots), as well as hyaluronidase and tyrosinase-inhibitory assays (up to 45.8 and 64.5% inhibition observed in the case of the SGB culture).
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Authors (10)
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Agata Krol
- Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk
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Adam Kokotkiewicz
- Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk
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Marcin Gorniak
- Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk
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Aleksandra M. Naczk
- Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk
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Jakub Gebalski
- Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University
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Filip Graczyk
- Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University
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Daniel Zaluski
- Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University
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Adam Bucinski
- Department of Biopharmacy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun
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Maria Luczkiewicz
- Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-023-33950-1
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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Scientific Reports
no. 13,
ISSN: 2045-2322 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Krol A., Kokotkiewicz A., Gorniak M., Naczk A. M., Zabiegała B., Gebalski J., Graczyk F., Zaluski D., Bucinski A., Luczkiewicz M.: Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots// Scientific Reports -,iss. 13/7141 (2023),
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-023-33950-1
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
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