PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum
Abstract
Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex Methods Two PCR assays were designed based on differences in the DNA fragment encoding b-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture. Result The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR. Conclusion We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory prax
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
no. 30,
edition 10,
pages 1819 - 1822,
ISSN: 0926-9959 - Language:
- English
- Publication year:
- 2016
- Bibliographic description:
- Kobylak N., Bykowska, B., Kurzyk E., Nowicki R., Brillowska-Dąbrowska A.: PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum// JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY. -Vol. 30, iss. 10 (2016), s.1819-1822
- DOI:
- Digital Object Identifier (open in new tab) 10.1111/jdv.13681
- Verified by:
- Gdańsk University of Technology
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