PCR test for Microsporum canis identification

Anna Brillowska-Dąbrowska; Ewelina Michałek; Ditte Marie Saunte; Sanne Sogaard Nielsen; Maiken Cavling Arendrup

PCR test for Microsporum canis identification

Abstract

Microsporum canis, for which the natural hosts are cats and dogs, is the most prevalent zoophilic agent causing tinea capitis and tinea corporis in humans. We present here a diagnostic PCR test for M. canis, since its detection and species identification is relevant to the choice of treatment and to the understanding of a probable source of infection. An M. canis-specific PCR was evaluated using 130 clinical isolates of dermatophytes (including M. canis [n = 15] and 13 other species), 10 yeast or mold isolates, 12 hair and skin samples from animals with or without experimental M. canis infection, and 35 patient specimens, including seven specimens positive for M. canis and 15 dermatophyte negative samples. All pure cultures, animal specimens and clinical samples with M. canis were detected by the PCR test, whereas none of the other fungal isolates or samples without M. canis was negative. This study indicates that the PCR test for M. canis identification applied directly to patient specimens or animal hair, as well as to clinical isolates had 100% specificity and sensitivity.

Authors (5)

Anna Brillowska-Dąbrowska dr hab. inż.
Ewelina Michałek
Ditte Marie Saunte
- Copenhagen University Hospital Department of Dermatology
Sanne Sogaard Nielsen
- Statens Serum Institut Unit of Mycology and PArasitology
Maiken Cavling Arendrup
- Statens Serum Institut Unit of Mycology and PArasitology

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Keywords

MICROSPORUM CANIS, DERMATOPHYTES, PCR, DIAGNOSTICS

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: MEDICAL MYCOLOGY no. 51, pages 576 - 579,
ISSN: 1369-3786
Language:: English
Publication year:: 2013
Bibliographic description:: Brillowska-Dąbrowska A., Michałek E., Saunte D., Nielsen S., Arendrup M.: PCR test for Microsporum canis identification// MEDICAL MYCOLOGY. -Vol. 51, nr. 6 (2013), s.576-579
Verified by:: Gdańsk University of Technology