Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation - Publication - Bridge of Knowledge

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Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation

Abstract

The 21st century has already brought us a plethora of new threats related to viruses that emerge in humans after zoonotic transmission or drastically change their geographic distribution or prevalence. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first spotted at the end of 2019 to rapidly spread in southwest Asia and later cause a global pandemic, which paralyzes the world since then. We have designed novel immunosensors targeting conserved protein sequences of the N protein of SARS-CoV-2 based on lab-produced and purified anti-SARS-CoV-2 nucleocapsid antibodies that are densely grafted onto various surfaces (diamond/gold/glassy carbon). Titration of antibodies shows very strong reactions up to 1:72 900 dilution. Next, we showed the mechanism of interactions of our immunoassay with nucleocapsid N protein revealing molecular recognition by impedimetric measurements supported by hybrid modeling results with both density functional theory and molecular dynamics methods. Biosensors allowed for a fast (in less than 10 min) detection of SARS-CoV-2 virus with a limit of detection from 0.227 ng/ml through 0.334 ng/ml to 0.362 ng/ml for glassy carbon, boron-doped diamond, and gold surfaces, respectively. For all tested surfaces, we obtained a wide linear range of concentrations from 4.4 ng/ml to 4.4 pg/ml. Furthermore, our sensor leads to a highly specific response to SARS-CoV-2 clinical samples versus other upper respiratory tract viruses such as influenza, respiratory syncytial virus, or Epstein-Barr virus. All clinical samples were tested simultaneously on biosensors and real-time polymerase chain reactions.

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DOI:
Digital Object Identifier (open in new tab) 10.1016/j.bios.2022.114222
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Category:
Articles
Type:
artykuły w czasopismach
Published in:
BIOSENSORS & BIOELECTRONICS no. 209,
ISSN: 0956-5663
Language:
English
Publication year:
2022
Bibliographic description:
Białobrzeska W., Ficek M., Dec B., Osella S., Trzaskowski B., Jaramillo-Botero A., Pierpaoli M., Rycewicz M., Dashkevich Y., Łęga T., Malinowska N., Cebula Z., Bigus D., Firganek D., Bięga E., Dziąbowska K., Brodowski M., Kowalski M., Panasiuk M., Gromadzka B., Żołędowska S., Nidzworski D., Pyrć K., Goddard Iii W. A., Bogdanowicz R.: Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation// BIOSENSORS & BIOELECTRONICS -Vol. 209, (2022), s.114222-
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.bios.2022.114222
Sources of funding:
Verified by:
Gdańsk University of Technology

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