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Photoinactivation of Staphylococcus aureus using protoporphyrin IX: the role of haem-regulated transporter HrtA
Abstract
Light- and photosensitiser-based antimicrobial photodynamic therapy is a very promising approach to the control of microbial infections. How the phenotypic features of a microorganism affect its response to photosensitiser-based photokilling represents an area of substantial research interest. To understand the mechanisms governing the phenomenon of a strain-dependent response to photodynamic inactivation (PDI), we analysed the possible role of the membrane-located haem transporter HrtA in Staphylococcus aureus. We used a S. aureus strains with an inactivated component of the haem-regulated transporter, HrtA, along with its wild-type counterpart to determine differences in PDI outcome and photosensitiser uptake between the studied isogenic strains. We observed that a lack of HrtA protein potentiates the phototoxic effect towards S. aureus but only when extracellular protoporphyrin IX is used. The observed effect may depend on the function of the HrtA transporter but is likely to result from changed membrane properties following the absence of the protein in the membrane. This indicates that disturbing the membrane properties is an attractive method for improving the efficacy of the photodynamic inactivation of microorganisms.
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Authors (6)
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Joanna Nakonieczna dr
- Międzyuczelniany Wydział Biotechnologii UG i GUMed Katedra Biotechnologii/Zakład Diagnostyki Molekularnej
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Monika Kossakowska-Zwierucho mgr inż.
- Międzyuczelniany Wydział Biotechnologii UG i GUMed Katedra Biotechnologii/Zakład Diagnostyki Molekularnej
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Michalina Filipiak
- Uniwersytet Gdański/Wydział Biologii Katedra Biologii Molekularnej
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Mariusz Grinholc
- Międzyuczelniany Wydział Biotechnologii UG i GUMed Katedra Biotechnologii/Zakład Diagnostyki Molekularnej
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00253-015-7145-5
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
no. 100,
edition 3,
pages 1393 - 1405,
ISSN: 0175-7598 - Language:
- English
- Publication year:
- 2016
- Bibliographic description:
- Nakonieczna J., Kossakowska-Zwierucho M., Filipiak M., Hewelt-Belka W., Grinholc M., Bielawski K.: Photoinactivation of Staphylococcus aureus using protoporphyrin IX: the role of haem-regulated transporter HrtA// APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. -Vol. 100, iss. 3 (2016), s.1393-1405
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00253-015-7145-5
- Verified by:
- Gdańsk University of Technology
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