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Abstract
This study evaluates the potential of rapid microbiological methods, traditionally used for food and environmental samples, to assess human milk (HM) quality in Human Milk Banks. Several methods, including a bioluminescence, the Micro Biological Survey (MBS) colorimetric method, triphenyltetrazolium chloride (TTC) reductase test, and flow cytometry (BacSomatic™️), were compared to the PN-EN ISO 4833–1:2013-12 reference method. Flow cytometry showed a strong correlation with the reference method for estimating bacterial counts in HM, indicating its potential as a fast alternative. Despite its speed − providing results in 10 min without pre-processing − flow cytometry showed some limitations suggesting it may not yet be suitable as a standalone tool. Other methods, such as TTC reduction test and bioluminescence, showed weaker correlations, indicating that external factors influence their accuracy, while flow cytometry is promising, further optimization is required before it can replace conventional methods.
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Authors (4)
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Izabela Drążkowska
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- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
INTERNATIONAL DAIRY JOURNAL
no. 166,
ISSN: 0958-6946 - Language:
- English
- Publication year:
- 2025
- Bibliographic description:
- Mazur K., Malinowska-Pańczyk E., Drążkowska I., Chmara M.: Rapid methods for microorganism quantification in human milk: Possibilities and evaluation// INTERNATIONAL DAIRY JOURNAL -,iss. 166 (2025),
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.idairyj.2025.106223
- Sources of funding:
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- Free publication
- Verified by:
- Gdańsk University of Technology
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