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Searching for the primary metabolic alterations of polycystic ovary syndrome by application of the untargeted metabolomics approach
Abstract
Despite a large number of studies, the pathogenesis of polycystic ovary syndrome (PCOS) still remains unexplained. In light of ambiguous observations reported in metabolomics, there is a need to carry out studies focusing on confirming the discriminating power of the proposed metabolomics biomarkers. Our research aimed to perform a validation study of metabolites detected in our previous study from serum samples, on the new set of samples obtained from PCOS women and healthy controls to confirm previously selected compounds. Additionally, the second biological matrix – urine – was used to get a more comprehensive insight into metabolic alterations. We applied two analytical techniques – gas chromatography and liquid chromatography coupled with mass spectrometry to analyze both serum and urine samples obtained from 35 PCOS patients and 35 healthy women. Thank to our approach, we identified and described a comprehensive set of metabolites altered in PCOS patients. Results of our study indicate increased steroid hormone synthesis, alteration in sphingo- and phospholipids metabolism, and disturbed fatty acids metabolism. Moreover, the citric acid cycle, γ-glutamyl cycle, vitamin B metabolism, and a few primary amino acids like tryptophan, phenylalanine, histidine, and alanine are altered.
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Authors (9)
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Anna Rajska
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Magdalena Buszewska-Forajta
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Szymon Macioszek
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Renata Wawrzyniak
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Andrzej Berger
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Agnieszka Kowalewska
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Dominik Rachon
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Michal Markuszewski
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.jpba.2023.115602
- License
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- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
no. 235,
ISSN: 0731-7085 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Rajska A., Buszewska-Forajta M., Macioszek S., Wawrzyniak R., Wityk P., Berger A., Kowalewska A., Rachon D., Markuszewski M.: Searching for the primary metabolic alterations of polycystic ovary syndrome by application of the untargeted metabolomics approach// JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS -, (2023), s.115602-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.jpba.2023.115602
- Sources of funding:
-
- własne
- Verified by:
- Gdańsk University of Technology
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