Search
The Effect of Marginal Zn2+ Excess Released from Titanium Coating on Differentiation of Human Osteoblastic Cells
Abstract
Composite coatings based on chitosan and zinc nanoparticles (ZnNPs) were successfully produced on Ti13Zr13Nb substrates by cathodic electrophoretic deposition (EPD). The unfavorable phenomenon of water electrolysis-induced nonuniformity was reduced by applying a low voltage (20 V) and a short deposition time (1 min). Surface analysis (roughness and hydrophilicity) reveals the potential of these coatings for enhancing cell attachment and bone-implant integration. However, there is a concern about adhesion and strength; therefore, incorporating ZnNPs shows promise for enhancing mechanical properties, suggesting opportunities for further optimization of the process. The aim of this work was to investigate whether Zn2+ released from coating yields overt cellular impairment. hFOB1.19 osteoblastic cells were used as a model in this study. A subtoxic, 0.125 mmol/L, Zn concentration did not cause significant negative changes in cultured osteoblastic cells, as there was no significant change in their viability and their mitochondrial metabolism. Moreover, the alkaline phosphatase and lactate dehydrogenase activities were aggravated. However, a high, over 0.175 mmol/L, Zn2+ concentration caused total cell death. This was caused by the inhibition of mitochondrial enzymes’ activities. Our data indicate that composite coatings releasing Zn2+ may be used as the differentiating factor toward osteoblastic cells.
Citations
-
0
CrossRef
-
0
Web of Science
-
0
Scopus
Authors (7)
-
Monika Sakowicz-Burkiewicz prof. dr hab.
-
Anna Ronowska dr hab. n. med.
Cite as
Full text
full text is not available in portal
Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
ACS Applied Materials & Interfaces
no. 16,
pages 48412 - 48427,
ISSN: 1944-8244 - Language:
- English
- Publication year:
- 2024
- Bibliographic description:
- Bartmański M., Pawłowski Ł., Knabe A., Mania S., Banach-Kopeć A., Sakowicz-Burkiewicz M., Ronowska A.: The Effect of Marginal Zn2+ Excess Released from Titanium Coating on Differentiation of Human Osteoblastic Cells// ACS Applied Materials & Interfaces -,iss. 36 (2024), s.48412-48427
- DOI:
- Digital Object Identifier (open in new tab) 10.1021/acsami.4c13529
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
seen 46 times