Abstract

Background.

Pancreatitis is the inflammation of the pancreas. The infl ammation may occur suddenly and resolve in a few days with the treatment in case of acute pancreatitis (AP). Chronic pancreatitis (CP) is a persistent inflammation of the pancreas that results in irreversible morphological changes and impairment of both exocrine and endocrine functions. Abdominal pain is a typical symptom in both acute and chronic pancreatitis. Therefore, the application of analgesics to this group of patients is a part of complex pharmacotherapy.

Material and methods. 
A simple, precise, specific, and accurate HPLC (High Performance Liquid Chromatography) method was developed 
for the determination of acetaminophen (AAP) in human plasma. The chromatography was set on XTerra® RP C18, 150×4.6 mm, 3.5 μm column. The UV detector was set at 254 nm for AAP and phenacetin (internal standard). The injection volume was 2 μL. The mobile phase comprised phosphate buffer pH 3.5 and methanol in the ratio of 80:20 with flow rate of 1 mL/min.

Results.

The calibration for AAP was linear in the range 1.0-30.0 μg/mL. Lower limit of quantifi cation (LLOQ) was 1.0 μg/mL. The results for quality control samples (QCs) at concentrations of 1, 6, 15, and 25 μg/mL were less than 15% indicating good intra- and inter-day precision. Blood samples from patients with chronic pancreatitis were collected within 8 h aft er the drug intravenous administration (1000 mg). Pharmacokinetic (PK) parameters for AAP were estimated by non-compartmental method.

Conclusion.

The proposed HPLC-UV method is suitable for routine analysis in patients. (Farm Współ 2017; 10: 3-10)

Authors (5)