Bioelectrochemical detection of COVID protein (RBD) on boron doped diamond - optimization of functionalziation parameters - Open Research Data - Bridge of Knowledge

Your account

login

Search

Bioelectrochemical detection of COVID protein (RBD) on boron doped diamond - optimization of functionalziation parameters

Description

The following dataset is a comprehensive overview of preparation of biofunctionalizaed BDD (boron-doped diamond) electrodes and their application in electrochemical sensing of COVID protein RBD. The specific receptor against RBD (Receptor Binding Domain) protein ACE-2 (Angiotensin Convertase Enzyme) is attached to the surface of BDD via a series of modification steps (diazonium electrografting, EDC/NHS linkers, and ACE-2 proteins). Functionalization parameters (linker concentrations, incubation times) are optimized towards optimal sensing performance. Influence of BSA adsorption after functionalizations on RBD detection is investigated. Stability and selectivity of the functionalziation is verified. Alternative route of functionalziation via his-tag was tested with negative results.

Dataset file

CZAPLA_functionalziations.zip
50.1 MB, S3 ETag 3467fe54a8aadc485008844d3422b5fc-1, downloads: 0
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5
download file CZAPLA_functionalziations.zip

File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution

Details

Year of publication:
2025
Verification date:
2025-03-13
Dataset language:
English
Fields of science:
  • materials engineering (Engineering and Technology)
DOI:
DOI ID 10.34808/hheg-zg78 open in new tab
Funding:
Verified by:
Gdańsk University of Technology

Keywords

Cite as

Authors

seen 6 times