Search
Determination of denaturation temperature of connective tissue proteins by viscometric measurements.
Description
Viscometric denaturation temperature of connective tissue proteins was determined by measuring viscosity with a Brookfield viscometer, using a LV SC4 - 18, 25 or LV3 spindle and shear rates from 50 to 110 s-1 (Fig.1). Freeze-dried protein samples were dissolved in 0.5 M acetic acid at 4 °C and concentration of 20 mg/mL. The solution was stirred with a magnetic stirrer for 2 hours. The measurement was made in the temperature range of 5 ÷ 40 °C with temperature increase equal 0.1 °C / minute. The volume of the sample used for the determination was dependend on the used spindle. Data allowed to determine the denaturation temperature of connective tissue proteins.
Fig.1. Brokfield rotational viscometer - model DV-III +.
Dataset file
Den.xlsx
16.6 kB,
S3 ETag
79209fe1cd10b1522628e61d3de82383-1,
downloads: 32
The file hash is calculated from the formula
Example script for calculation:
https://github.com/antespi/s3md5
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
open in new tab
CC BY-NC-SANon-commercial - Share-alike - Raw data:
- Data contained in dataset was not processed.
- Software:
- EXEL
Details
- Year of publication:
- 2020
- Verification date:
- 2020-12-17
- Dataset language:
- English
- Fields of science:
-
- chemical sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/zmet-9y73 open in new tab
- Verified by:
- Gdańsk University of Technology
Keywords
Cite as
Authors
seen 114 times