Investigation of the C-1311 glucuronidation: an electrochemical approach - Open Research Data - Bridge of Knowledge

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Investigation of the C-1311 glucuronidation: an electrochemical approach

Description

This study was undertaken to investigate the glucuronidation of the compound C-1311 (5-diethylaminoethylamino-8-hydroxyimidazoacridinone – the model anticancer acridine derivative) using electrochemistry/mass spectrometry (EC/MS) as a complementary technique to in vitro (liver microsomes) and in silico approaches.

For the EC/MS experiment the RoxyTM system (Antec Scientific, The Netherlands) equipped with an electrochemical thin-layer ReactorCellTM connected directly to a 6470 Triple Quad LC/MS with an AJS (Agilent Jet Stream) ESI interface (Agilent Technologies, USA) was used. The ReactorCellTM was supplied to a disc glassy carbon working electrode (ϕ =8 mm; A = 0.502 cm2) and a HyREF palladium-hydrogen (Pd/H2) pseudo reference electrode. The upper half of the electrochemical cell block made of a chemically inert and physically resistant polyether ether ketone (PEEK; 30% carbon fiber-reinforced) was employed as a counter electrode.

Generation of C-1311 glucuronides:

  • Sample: 10 μM C-1311 solution in 10 mM ammonium formate (pH ~ 7.4) and methanol (1:1, v/v) (electrolyte solution)
  • Conjugation reaction: addition of 100 μM glucuronic acid solution in electrolyte solution after the ReactorCellTM via 100 μL T-piece to conjugate C-1311 and/or phase I metabolites
  • Flow rate: 20 μL/min
  • Potential: from 0 to 2500 mV (at 10 mV/s)
  • Reaction temperature: room temperature

EC/MS made it possible to detect three potential 8-O-glucuronides (predicted by XenoSite Reactivity Predictor) derived from C-1311 (m/z 351) and its derivatives – products of C-1311 side chain degradation (corresponding ions at m/z 278 and m/z 323).

Dataset file

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Details

Year of publication:
2024
Verification date:
2024-05-31
Creation date:
2024
Dataset language:
English
Fields of science:
  • chemical sciences (Natural sciences)
DOI:
DOI ID 10.34808/ncw3-kx93 open in new tab
Verified by:
Gdańsk University of Technology

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