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The impact of QDgreen, QDgreen−β−CD−FA, β−CD, C−2028, β−CD(C−2028), QDgreen−C−2028, and QDgreen−β−CD(C−2028)−FA nanoconjugates on lysosomal content in the cancer (H460, Du-145, LNCaP) and normal (MRC-5, PNT1A) cells
Description
The impact of QDgreen, QDgreen−β−CD−FA, β−CD, C−2028, β−CD(C−2028), QDgreen−C−2028, and QDgreen−β−CD(C−2028)−FA nanoconjugates on lysosomal content in the cancer (H460, Du-145, LNCaP) and normal (MRC-5, PNT1A) cells was performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). To explore the influence of compounds on lysosomal content in the cells LysoTracker Red DND-99 (Invitrogen, USA) was added to a final concentration of 100 nM for 15 min at 37 °C. The imaging conditions were: QDgreen (λex = 300 nm, λem = 543 nm), C−2028 (λex = 528 nm, λem = 553 nm), and LysoTracker Red DND-99 (λex = 578 nm, λem = 700 nm). The Mean Fluorescence Intensity (MFI) of LysoTracker Red DND-99 from the images was performed with ImageJ software (version 1.8.0., Wisconsin, Madison, USA). The scale bar is 20 µm.
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
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CC BY-NC-SANon-commercial - Share-alike
Details
- Year of publication:
- 2022
- Verification date:
- 2022-03-22
- Dataset language:
- English
- Fields of science:
-
- chemical sciences (Natural sciences)
- biomedical engineering (Engineering and Technology)
- DOI:
- DOI ID 10.34808/a2v2-c880 open in new tab
- Funding:
- Verified by:
- Gdańsk University of Technology
Keywords
References
- dataset The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer LNCaP cells
- dataset The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to normal PNT1A cells
- dataset The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer H460 cells
- dataset The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer Du-145 cells
- dataset The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to normal MRC-5 cells
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