The mechanism of internalization study of QDgreen−CD−FA−C−2028 conjugate at IC80 value to cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells - Open Research Data - Bridge of Knowledge

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The mechanism of internalization study of QDgreen−CD−FA−C−2028 conjugate at IC80 value to cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells

Description

The influence of different endocytosis inhibitors on the internalization of QDgreen−CD−FA−C−2028 conjugate at IC80 value in cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells. First, the cells were preincubated with: drug-free medium (no inhibitor), at 4 °C, 5 µM Cytochalasin D, 30 µM Amiloride, 80 µM Dynasore, 25 µM Pitstop 2 and 1.5 µM Filipin III for 30 min, followed by further incubation with QDgreen−CD−FA−C−2028 for 4 h. The internalization of QDgreen−CD−FA−C−2028 in cells was explored by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT). Based on the fluorescence properties of these compounds, green and orange fluorescence are representative for QDgreen and C−2028, respectively. The imaging conditions were: QDgreen (excitation 300 nm, emission 543 nm), C−2028 (excitation 528 nm, emission 553 nm). The scale bar is 20 μm.

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The mechanism of internalization study of QDgreen−CD−FA−C−2028 conjugate at IC80 value to cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells.zip
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License:
Creative Commons: by-nc-sa 4.0 open in new tab
CC BY-NC-SA
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Year of publication:
2021
Verification date:
2021-12-09
Dataset language:
English
Fields of science:
  • chemical sciences (Natural sciences)
  • biomedical engineering (Engineering and Technology)
DOI:
DOI ID 10.34808/zsy0-az13 open in new tab
Funding:
Verified by:
Gdańsk University of Technology

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