The PolarScreen™ Estrogen Receptor Competitor Assays for determination of estradiol equivalent concentrations (EEQs) in sewage and drinking water samples.

Description

Municipal waste waters are one of the main sources of estrogenic compounds in aquatic environments. Feminization of fish downstream of Waste Water Treatment Plants (WWTPs) discharges has been observed worldwide. Some estrogenic chemicals, particularly steroid estrogens, are known to cause disruption of the endocrine system of fishes and abnormalities of the reproductive tract in ng/L concentrations, which commonly occur in aquatic environment worldwide.

In vitro assays are broadly used tools to evaluate the estrogenic activity in Waste Water Treatment Plant (WWTP) effluents. Estrone, 17beta-estradiol, 17alfa-ethinylestradiol and to some extent, estriol have been shown to be responsible for the majority of in vitro estrogenic activity of municipal WWTP effluents.

In this study there was evaluated the estrogenicity of the samples in terms of their estradiol equivalent concentrations (EEQs) using The PolarScreen™ Estrogen Receptor Competitor Assays, green.

Influent and effluent water samples were collected at the wast water treatment plant (WWTP) and drinking water was taken from hausehold water supply. All samples were collected to glass amber bottles filled up to the brim. The samples were immediately transported to the laboratory, stored at 4oC and analyzed within 24h.

Dataset file

EEQ.xls

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CC BY-NC-SA

Non-commercial - Share-alike
Software:: Excel

Details

Year of publication:

2020

Verification date:

2020-12-17

Dataset language:

English

Fields of science:

chemical sciences (Natural sciences)
Earth and related environmental sciences (Natural sciences)

DOI:

10.34808/x4y7-ak57

Verified by:

Gdańsk University of Technology

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