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Toxoplasma gondii recombinant chimeric antigens - IgG and IgM ELISAs - human serum samples

Description

This study presents an evaluation of tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG1, SAG2, GRA1, GRA2, MIC1, MAG1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG1-MIC1-MAG1-GRA2, SAG2-GRA1-ROP1-GRA2, SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were tested for their reactivity with specific IgM and IgG antibodies from sera of experimentally infected mice and humans with T. gondii infection using an enzyme-linked immunosorbent assay (ELISA). Serum samples from patients with acquired T. gondii infection and sera from seronegative individuals were examined. The reactivity of chimeric antigens with antibodies generated during T. gondii invasion was measured and compared to the results obtained in assays based on whole-cell Toxoplasma antigen. Chimeric proteins proved effective in differentiation between T. gondii-infected and uninfected individuals (100% sensitivity and specificity in the IgG ELISAs) which shows their potential usefulness as a replacements for TLA in standardized commercial tests for the serodiagnosis of toxoplasmosis.

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License:
Creative Commons: by 4.0 open in new tab
CC BY
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Details

Year of publication:
2019
Verification date:
2020-12-17
Dataset language:
Polish
Fields of science:
  • biological sciences (Natural sciences)
  • medical sciences (Medical and Health Sciences )
DOI:
DOI ID 10.34808/w7qp-2j94 open in new tab
Funding:
Ethical papers:
The sera were collected as part of the project entitled "Toxoplasmosis—facts and myths. Educational initiative raising social awareness about the infection with protozoan Toxoplasma gondii", foundation "Our Children", funded from the Civil Initiatives Fund of the Ministry of Labor and Social Policy (FIO 2008, contract No. 813).
Verified by:
Gdańsk University of Technology

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