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A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties
Abstract
A psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is optimally active at pH 6.5-7.5 and 40°C, and exhibits 38-44% of the maximum activity at 5-10°C. This enzyme is activated by Mg2+ and Ca2+ ions, and inhibited by Mn2+, Ni2+ and Co2+ ions. The properties of Arthrobacter sp. S3* β-galactosidase suggests that this enzyme could be useful for low-lactose milk production.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie indeksowanym TR Master Journal List
- Published in:
-
Acta Biochimica Polonica
no. 57,
ISSN: 0001-527X - Language:
- English
- Publication year:
- 2010
- Bibliographic description:
- Wanarska M., Ługowski Ł., Kur J.: A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties// Acta Biochimica Polonica. -Vol. 57., nr. Suppl 3 (2010),
- Verified by:
- Gdańsk University of Technology
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