A Novel Method of Endotoxins Removal from Chitosan Hydrogel as a Potential Bioink Component Obtained by CO2 Saturation - Publication - Bridge of Knowledge

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A Novel Method of Endotoxins Removal from Chitosan Hydrogel as a Potential Bioink Component Obtained by CO2 Saturation

Abstract

The article presents a new approach in the purification of chitosan (CS) hydrogel in order to remove a significant amount of endotoxins without changing its molecular weight and viscosity. Two variants of the method used to purify CS hydrogels from endotoxins were investigated using the PyroGene rFC Enzymatic Cascade assay kit. The effect of the CS purification method was assessed in terms of changes in the dynamic viscosity of its hydrogels, the molecular weight of the polymer, microbiological purity after refrigerated storage and cytotoxicity against L929 cells based on the ISO 10993-5:2009(E) standard. The proposed purification method 1 (M1) allows for the removal of significant amounts of endotoxins: 87.9–97.6% in relation to their initial concentration in the CS hydrogel without affecting the solution viscosity. Moreover, the final solutions were sterile and microbiologically stable during storage. The M1 purification method did not change the morphology of the L929 cells.

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DOI:
Digital Object Identifier (open in new tab) 10.3390/ijms23105505
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Category:
Articles
Type:
artykuły w czasopismach
Published in:
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES no. 23,
ISSN: 1661-6596
Language:
English
Publication year:
2022
Bibliographic description:
Banach-Kopeć A., Mania S., Pilch J., Augustin E., Gabriel I., Tylingo R.: A Novel Method of Endotoxins Removal from Chitosan Hydrogel as a Potential Bioink Component Obtained by CO2 Saturation// INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -Vol. 23,iss. 10 (2022), s.5505-
DOI:
Digital Object Identifier (open in new tab) 10.3390/ijms23105505
Sources of funding:
  • Minigrant nr 032408T103 Wydział Chemiczny PG
Verified by:
Gdańsk University of Technology

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