A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease

Maria Tarnawska; Karolina Dorniak; Mariusz Franciszek Kaszubowski; Marian Dudziak; Marcin Hellmann

doi:10.5603/cj.a2017.0096

A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease

Abstract

Background: Endothelial dysfunction is one of the earliest vascular manifestations in the pathogenesis of cardiovascular disease. Noninvasive, simple and inexpensive methods of endothelial function assessment are therefore needed. Methods: Microvascular endothelial function was assessed in coronary artery disease (CAD) patients by flow mediated skin fluorescence (FMSF), based on measurements of NADH fluorescence intensity during brachial artery occlusion (ischemic response [IRmax]) and immediately after occlusion (hyperemic response [HRmax]). Additionally, plasma levels of asymmetric dimethylarginine (ADMA) and endothelin-1 (ET-1) were measured to assess the association between biochemical markers and microvascular function evaluated in vivo by FMSF. Results: A significant inverse correlation was found between ADMA levels and hyperemic response (r = –0.534, p = 0.003), while ET-1 levels were inversely related to the ischemic response (r= –0.575, p = 0.001). Both IR and HR were found lowest in patients with advanced CAD and diabetes. When the repeatability of the method was tested, the intraclass correlation coefficient for IRmax and HRmax were 0.985 (p < 0.001) and 0.914 (p < 0.001), respectively. Moreover, in Bland and Altman analysis, both variables IRmax and HRmax showed good agreement in repeated measurements. Conclusions: In this pilot study, it was demonstrated that NADH fluorescence measured by FMSF device in CAD patients was associated with established plasma endothelial markers, and that both ischemic and hyperemic response were blunted in patients with advanced disease and diabetes. Furthermore, FMSF device showed excellent repeatability and good agreement for repeated measurements. However, further study is warranted to confirm these results in a larger patient cohort.

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DOI:: Digital Object Identifier (open in new tab) 10.5603/CJ.a2017.0096
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Keywords

BRACHIAL ARTERY OCCLUSION

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: Cardiology Journal no. 25, pages 120 - 127,
ISSN: 1897-5593
Language:: English
Publication year:: 2018
Bibliographic description:: Tarnawska M., Dorniak K., Kaszubowski M. F., Dudziak M., Hellmann M.: A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease// Cardiology Journal. -Vol. 25, iss. 1 (2018), s.120-127
DOI:: Digital Object Identifier (open in new tab) 10.5603/cj.a2017.0096
Verified by:: Gdańsk University of Technology