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A Three-Step Approach to Estimation of Reduction Potentials of Natural Mixtures of Antioxidants Based on DPPH Test; Illustration for Catechins and Cocoa
Abstract
The aim of this study is to propose a methodology to assess electrochemical properties of complex mixtures of antioxidants, such as plant extracts, based on the results of simple and popular DPPH test. The first, most difficult step, involves determinations of standard reduction potentials (E0) for the series of purified compounds (here catechins). The next step is the calculation of stoichiometric values (n10) based on the results of DPPH test for the same compounds. Finally, a correlation equation is formulated, which is then employed to estimate “cumulative reduction potential” (Ec) for the mixture of interest (here cocoa) using DPPH test results.
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- Category:
- Articles
- Type:
- publikacja w in. zagranicznym czasopiśmie naukowym (tylko język obcy)
- Published in:
-
Proceedings
no. 11,
pages 1 - 5,
ISSN: - Language:
- English
- Publication year:
- 2019
- Bibliographic description:
- Kusznierewicz B., Baranowska M., Suliborska K., Chrzanowski W., Namieśnik J., Bartoszek-Pączkowska A.. A Three-Step Approach to Estimation of Reduction Potentials of Natural Mixtures of Antioxidants Based on DPPH Test; Illustration for Catechins and Cocoa. Proceedings, 2019, Vol. 11, iss. 1, s.1-5
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/proceedings2019011004
- Bibliography: test
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- Baranowska, M.; Suliborska, K.; Chrzanowski, W.; Kusznierewicz, B.; Namieśnik, J.; Bartoszek, A. The relationship between standard reduction potentials of catechins and biological activities involved in redox control. Redox Biol. 2018, 17, 355-366, doi:10.1016/j.redox.2018.05.005. open in new tab
- Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical. Free Radic. Biol. Med. 1999, 26, 1231-1237, doi:10.1016/S0891-5849(98)00315-3. open in new tab
- Goupy, P.; Dufour, C.; Loonis, M.; Dangles, O. Quantitative kinetic analysis of hydrogen transfer reactions from dietary polyphenols to the DPPH radical. J. Agric. Food Chem 2003, 51, 615-622, doi:10.1021/jf025938l. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). open in new tab
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- Gdańsk University of Technology
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