A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms - Publication - Bridge of Knowledge

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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

Abstract

ABSTRACT Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.

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Category:
Articles
Type:
artykuły w czasopismach recenzowanych i innych wydawnictwach ciągłych
Published in:
Roczniki Państwowego Zakładu Higieny no. 68, pages 247 - 251,
ISSN: 0035-7715
Language:
English
Publication year:
2017
Bibliographic description:
Wołoszyn A., Kotłowski R.: A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms// Roczniki Państwowego Zakładu Higieny. -Vol. 68., nr. 3 (2017), s.247-251
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  1. Dellaporta S.L., Wood J, Hicks J.B.: A plant DNA minipreparation: Version II. Plant Molecular Biology Reporter 1983;1(4):19-21. open in new tab
  2. Gausterer C., Penker M., Krisai-Greilhuber I., Stein C., Stimpfl T.: Rapid genetic detection of ingested Amanita phalloides. Forensic Sci Int Genet 2014;9:66-71. open in new tab
  3. Hallen H.E., Luo H., Scott-Craig J.S., Walton J.D.: Gene family encoding the major toxins of lethal Amanita mushrooms. Proc Natl Acad Sci U S A 2007;104(48):19097-101. open in new tab
  4. Hammer Ø. Harper D.A.T. Ryan P.D.: PAST: Paleontological statistics software package for education and data analysis. Paleontologia Electronica 2001;4(1):9. open in new tab
  5. Maeta K., Ochi T., Tokimoto K., Shimomura N., Maekawa N., Kawaguchi N., Nakaya M., Kitamoto Y., Aimi T.: Rapid species identification of cooked poisonous mushrooms by using real-time PCR. Appl Environ Microbiol 2008 May;74(10):3306-9. open in new tab
  6. Kapala M., Nowacka A., Kicka M., Rakowski M.: Mushroom (fungi) poisonings investigated at the regional centre of acute poisoning, institute of occupational medicine and environmental health, Sosnowiec, Poland. Problems of Forensic Sciences 2008;LXXV:282-293.
  7. Kotłowski R.: Mashroom toxins. In: Witczak A., Sikorski Z.E. eds. Toxins and other harmful compounds in foods. New York, CRC Press Tylor & Francis Group, 2017.
  8. Kotlowski R., Myjak P., Kur J.: Specific detection of Amanita phalloides mycelium and spores by PCR amplification of the gpd (glyceraldehyde-3-phosphate dehydrogenase) gene fragment. J Food Biochem 2000;24(3):201-212. open in new tab
  9. Kowalczyk M., Sekuła A., Mleczko P., Olszowy Z., Kujawa A., Zubek S., Kupiec T.: Practical aspects of genetic identification of hallucinogenic and other poisonous mushrooms for clinical and forensic purposes. Croat Med J 2015;56(1):32-40. open in new tab
  10. Reports on cases of infectious diseases and poisonings in Poland -2000-2015. Available http://wwwold. pzh.gov.pl/oldpage/epimeld/index_p.html (Accesed 27.06.2017). open in new tab
  11. Tsuruda S., Akaki K., Hiwaki H., Suzuki A., Akiyama H.: Multiplex real-time PCR assay for simultaneous detection of Omphalotus guepiniformis and Lentinula edodes. Biosci Biotechnol Biochem 2012;76(7):1343-9. open in new tab
  12. Vilgalys R., Gonzalez D.: Organisation of ribosomal DNA in the basidiomycete Thanatephorus praticola. Current Genetics 1990;18:277-80. open in new tab
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Gdańsk University of Technology

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