Advantageous conditions of saccharification of lignocellulosic biomass for biofuels generation via fermentation processes - Publication - MOST Wiedzy


Advantageous conditions of saccharification of lignocellulosic biomass for biofuels generation via fermentation processes


Processing of lignocellulosic biomass includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purifcation prior to biofuel generation via anaerobic digestion. The microorganisms involved in the fermentation metabolize only simple molecules, i.e., monosugars which can be obtained by carrying out the degradation of complex polymers, the main component of lignocellulosic biomass. The object of this paper was to evaluate the saccharifcation conditions and identify the process parameters that should be applied to improve the saccharifcation efciency of lignocellulosic biomass, defned as the simple sugars concentration, which was considered as a crucial parameter for hydrogen generation via dark fermentation. Drawing global conclusions about the occurring changes in the biomass requires learning about the nature of the biomass structure and composition at diferent stages of the process. Therefore, techniques for analysis, as FTIR, HPLC and SEM were applied. The experiment was planned employing Box–Behnken design. The advantageous operating conditions and the composition of saccharifcation enzymatic cocktail were identifed and their values occurred similar in the applied border conditions for all tested biomass types. Analysis of the intermediate solid and liquid streams generated during the pre-treatment procedure revealed several structural and compositional changes in the biomass.


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artykuły w czasopismach
Published in:
CHEMICAL PAPERS no. 74, pages 1199 - 1209,
ISSN: 0366-6352
Publication year:
Bibliographic description:
Kucharska K., Słupek E., Cieśliński H., Kamiński M.: Advantageous conditions of saccharification of lignocellulosic biomass for biofuels generation via fermentation processes// CHEMICAL PAPERS -Vol. 74, (2020), s.1199-1209
Digital Object Identifier (open in new tab) 10.1007/s11696-019-00960-1
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