An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum

Adrian Koterwa; Magdalena Bojko; Jacek Ryl; Krzysztof Łukaszuk; Kornelia Kozłowska; Wiktor Sieklicki; Sylwia Rodziewicz-Motowidło; Pawel Niedzialkowski

doi:10.1002/elan.202300095

An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum

Abstract

This work describes the modification of a gold electrode to create an electrochemical biosensor capable of detecting human chorionic gonadotropin (hCG). The biosensor was obtained by modifying the gold electrode with cysteamine and oligopeptide (PPLRINRHILTR). The modification steps of the gold electrode were confirmed by cyclic voltammetry (CV) and impedance electrochemical spectroscopy (EIS) measurements. The conducted EIS experiments in 0.01 M PBS, pH 7.4 confirm that the biosensor exhibits sensitivity towards hCG in a range of concentrations from 1×10−12 to 1×10−7 M (0.5 mIU/mL – 50 000 mIU/mL) to solutions with a detection limit of 1. 91×10−14 M (0.0095 mIU/mL). The effectiveness of the investigated biosensor was also investigated in human serum. The EIS comparative investigations were performed in human serum containing a concentration of 1×10−12 M (0.5 mIU/mL) hCG and in human serum where the hCG was added. The obtained results indicate that the investigated biosensor is selective for the presence of hCG hormone in the human serum.

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Authors (8)

Adrian Koterwa
Magdalena Bojko
Jacek Ryl dr hab. inż.
Krzysztof Łukaszuk
Kornelia Kozłowska
Wiktor Sieklicki dr inż.
Sylwia Rodziewicz-Motowidło dr hab.
Pawel Niedzialkowski

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Keywords

ELECTROCHEMICAL DETECTION, GOLD ELECTRODE FUNCTIONALIZATION, HUMAN CHORIONIC GONADOTROPIN (HCG), HUMAN SERUM

Details

Category:

Articles

Type:

artykuły w czasopismach

Published in:

ELECTROANALYSIS no. 35, pages 1 - 9,
ISSN: 1040-0397

Language:

English

Publication year:

2023

Bibliographic description:

Koterwa A., Bojko M., Ryl J., Łukaszuk K., Kozłowska K., Sieklicki W., Rodziewicz-Motowidło S., Niedzialkowski P.: An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum// ELECTROANALYSIS -,iss. 11 (2023),

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