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An optimal designed experiment for the alkaline hydrolysis of feather keratin

Abstract

Feathers, burdensome waste from the poultry industry, can be a cheap source of keratin, a protein with excellent physico- chemical, biological, and mechanical properties. Acid and alkaline hydrolyses are usually adopted for isolation of keratin from its natural resources. This study aimed at assessing the statistically significant effect of input variables in the alkaline hydrolysis of keratin from chicken feathers on the process yield and on the molecular weight of peptides obtained. The effect of the volume ratio of 1M NaOH to the feathers’ mass, the hydrolysis time, and the shaking speed of the reaction mixture on the process yield were analyzed. The use of statistical analysis at the design step of experiment allowed reducing the trial number from 27 to 9. Among the input variables analyzed, only the volume ratio of 1M NaOH to the feathers’ mass had a significant effect on the process yield, while none of them significantly affected the molecular weight of the peptides obtained. All hydrolysates were dominated by two peptides’ fractions, with molecular weights of ca. 130 and 250 kDa, and mixture of many peptides of weight close to 10 kDa and smaller. Alkaline hydrolysis of feather keratin yielded protein hydrolysates soluble over a wide pH range.

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DOI:
Digital Object Identifier (open in new tab) 10.1007/s11356-021-17649-2
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Details

Category:
Articles
Type:
artykuły w czasopismach
Published in:
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH no. 29, pages 24145 - 24154,
ISSN: 0944-1344
Language:
English
Publication year:
2022
Bibliographic description:
Dąbrowska M., Sommer A., Sinkiewicz I., Taraszkiewicz A., Staroszczyk H.: An optimal designed experiment for the alkaline hydrolysis of feather keratin// ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH -Vol. 29,iss. 1 (2022), s.24145-24154
DOI:
Digital Object Identifier (open in new tab) 10.1007/s11356-021-17649-2
Verified by:
Gdańsk University of Technology

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