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Analysis of multi-class pharmaceuticals in fish tissues by ultra-high-performance liquid chromatography tandem mass spectrometry
Abstract
A new sensitive method based on pressurized liquid extraction (PLE) and purification by gel permeation chromatography (GPC) prior to ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) was developed for the determination in fish homogenate, liver and muscle of twenty pharmaceuticals compounds and metabolites from seven commonly used therapeutic families. An extensive matrix effect evaluation was performed in order to select the best approach when analyzing such complex matrices. Limits of detection (MDLs) for the target compounds were in the range of 0.03–0.50 ng/g for fish homogenate, 0.01–0.42 ng/g for fish muscle, and 0.08–0.98 ng/g for fish liver. The method was applied to fish tissues of eleven fish species from four heavily impacted Mediterranean rivers. Nine compounds from five therapeutic families were measured at concentrations higher than MDLs. Highest levels were found in trout liver, with a maximum concentration of 18 ng/g for carbamazepine, whereas the most ubiquitous compound was diclofenac.
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Authors (5)
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Belinda Huerta
- Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain
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Meritxell Gros
- Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain
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Sara Rodriguez-Mozaz
- Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain
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Damia Barcelo
- Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain
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Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF CHROMATOGRAPHY A
pages 63 - 72,
ISSN: 0021-9673 - Language:
- English
- Publication year:
- 2013
- Bibliographic description:
- Huerta B., Jakimska-Nagórska A., Gros M., Rodriguez-Mozaz S., Barcelo D.: Analysis of multi-class pharmaceuticals in fish tissues by ultra-high-performance liquid chromatography tandem mass spectrometry// JOURNAL OF CHROMATOGRAPHY A. -, nr. 1288 (2013), s.63-72
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.chroma.2013.03.001
- Verified by:
- Gdańsk University of Technology
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