Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration
Abstract
Bacterial infections due to bone replacement surgeries require modifications of bone cement with antibacterial components. This study aimed to investigate whether the incorporation of gentamicin or nanometals into bone cement may reduce and to what extent bacterial growth without the loss of overall cytocompatibility and adverse effects in vitro. The bone cement Cemex was used as the base material, modified either with gentamicin sulfate or nanometals: Silver or copper. The inhibition of bacterial adhesion and growth was examined against five different bacterial strains along with integrity of erythrocytes, viability of blood platelets, and dental pulp stem cells. Bone cement modified with nanoAg or nanoCu revealed greater bactericidal effects and prevented the biofilm formation better compared to antibiotic-loaded bone cement. The cement containing nanoAg displayed good cytocompatibility without noticeable hemolysis of erythrocytes or blood platelet disfunction and good viability of dental pulp stem cells (DPSC). On the contrary, the nanoCu cement enhanced hemolysis of erythrocytes, reduced the platelets aggregation, and decreased DPSC viability. Based on these studies, we suggest the modification of bone cement with nanoAg may be a good strategy to provide improved implant fixative for bone regeneration purposes.
Citations
-
3 9
CrossRef
-
0
Web of Science
-
3 9
Scopus
Authors (7)
Cite as
Full text
- Publication version
- Accepted or Published Version
- License
- open in new tab
Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
Nanomaterials
no. 9,
pages 1 - 18,
ISSN: 2079-4991 - Language:
- English
- Publication year:
- 2019
- Bibliographic description:
- Wekwejt M., Michno A., Truchan K., Pałubicka A., Świeczko-Żurek B., Osyczka A., Zieliński A.: Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration// Nanomaterials. -Vol. 9, iss. 8 (2019), s.1-18
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/nano9081114
- Bibliography: test
-
- Radha, G.; Balakumar, S.; Venkatesan, B.; Vellaichamy, E. A novel nano-hydroxyapatite-PMMA hybrid scaffolds adopted by conjugated thermal induced phase separation (TIPS) and wet-chemical approach: Analysis of its mechanical and biological properties. Mater. Sci. Eng. C 2017, 73, 164-172.
- Vaishya, R.; Chauhan, M.; Vaish, A. Bone cement. J. Clin. Orthop. Trauma 2013, 4, 157-163. [CrossRef] [PubMed] open in new tab
- Curatolo, C.J.; Anderson, M.R. Bone cement implantation syndrome. In Decision-Making in Orthopedic and Regional Anesthesiology: A Case-Based Approach; open in new tab
- Anderson, M.R., Wilson, S.H., Rosenblatt, M.A., Eds.; Cambridge University Press: Cambridge, UK, 2015; pp. 118-122.
- Slane, J.; Vivanco, J.; Ebenstein, D.; Squire, M.; Ploeg, H.L. Multiscale characterization of acrylic bone cement modified with functionalized mesoporous silica nanoparticles. J. Mech. Behav. Biomed. Mater. 2014, 37, 141-152. [CrossRef] [PubMed] open in new tab
- Li, H.; Gu, J.; Shah, L.A.; Siddiq, M.; Hu, J.; Cai, X.; Yang, D. Bone cement based on vancomycin loaded mesoporous silica nanoparticle and calcium sulfate composites. Mater. Sci. Eng. C 2015, 49, 210-216. [CrossRef] [PubMed] open in new tab
- He, Z.; Zhai, Q.; Hu, M.; Cao, C.; Wang, J.; Yang, H.; Li, B. Bone cements for percutaneous vertebroplasty and balloon kyphoplasty: Current status and future developments. J. Orthop. Transl. 2015, 3, 1-11. [CrossRef] open in new tab
- Hoess, A.; López, A.; Engqvist, H.; Ott, M.K.; Persson, C. Comparison of a quasi-dynamic and a static extraction method for the cytotoxic evaluation of acrylic bone cements. Mater. Sci. Eng. C 2016, 62, 274-282. [CrossRef] open in new tab
- Robo, C.; Hulsart-Billström, G.; Nilsson, M.; Persson, C. In vivo response to a low-modulus PMMA bone cement in an ovine model. Acta Biomater. 2018, 72, 362-370. [CrossRef] open in new tab
- Inzana, J.A.; Schwarz, E.M.; Kates, S.L.; Awad, H.A. Biomaterials approaches to treating implant-associated osteomyelitis. Biomaterials 2016, 81, 58-71. [CrossRef] open in new tab
- Cenni, E.; Granchi, D.; Vancini, M.; Pizzoferrato, A. Platelet release of transforming growth factor-β and β-thromboglobulin after in vitro contact with acrylic bone cements. Biomaterials 2002, 23, 1479-1484. [CrossRef] open in new tab
- Matus, F.; Vilos, C.; Cisterna, B.A.; Fuentes, E.; Palomo, I. Nanotechnology and primary hemostasis: Differential effects of nanoparticles on platelet responses. Vasc. Pharmacol. 2018, 101, 1-8. [CrossRef] open in new tab
- Fröhlich, E. Hemocompatibility of inhaled environmental nanoparticles: Potential use of in vitro testing. J. Hazard. Mater. 2017, 336, 158-167. [CrossRef] [PubMed] open in new tab
- Przekora, A. The summary of the most important cell-biomaterial interactions that need to be considered during in vitro biocompatibility testing of bone scaffolds for tissue engineering applications. Mater. Sci. Eng. C 2019, 97, 1036-1051. [CrossRef] [PubMed] open in new tab
- Zamborsky, R.; Svec, A.; Bohac, M.; Kilian, M.; Kokavec, M. Infection in bone allograft transplants. Exp. Clin. Transplant. 2016, 14, 484-490. [PubMed] open in new tab
- Miola, M.; Bistolfi, A.; Valsania, M.C.; Bianco, C.; Fucale, G.; Verné, E. Antibiotic-loaded acrylic bone cements: An in vitro study on the release mechanism and its efficacy. Mater. Sci. Eng. C 2013, 33, 3025-3032. [CrossRef] [PubMed] open in new tab
- Ferreira, M.; Rzhepishevska, O.; Grenho, L.; Malheiros, D.; Gonçalves, L.; Almeida, A.J.; Jordão, L.; Ribeiro, I.; Ramstedt, M.; Gomes, P.; et al. Levofloxacin-loaded bone cement delivery system: Highly effective against intracellular bacteria and Staphylococcus aureus biofilms. Int. J. Pharm. 2017, 532, 241-248. [CrossRef] open in new tab
- Nanomaterials 2019, 9, 1114 open in new tab
- Paz, E.; Sanz-Ruiz, P.; Abenojar, J.; Vaquero-Martín, J.; Forriol, F.; Del Real, J.C. Evaluation of Elution and Mechanical Properties of High-Dose Antibiotic-Loaded Bone Cement: Comparative "In Vitro" Study of the Influence of Vancomycin and Cefazolin. J. Arhroplasty 2015, 30, 1423-1429. [CrossRef] [PubMed] open in new tab
- Frutos, G.; Pastorr, J.Y.; Martinez, N.; Virto, M.R.; Torrado, S. Influence of lactose addition to gentamicin-loaded acrylic bone cement on the kinetics of release of the antibiotic and the cement properties. Acta Biomater. 2010, 6, 804-811. [CrossRef] open in new tab
- Rodrigues, G.R.; López-Abarrategui, C.; de la Serna Gómez, I.; Dias, S.D.; Otero-González, A.J.; Franco, O.L. Antimicrobial magnetic nanoparticles based-therapies for controlling infectious diseases. Int. J. Pharm. 2019, 555, 356-367. [CrossRef] open in new tab
- Zheng, K.; Setyawati, M.I.; Leong, D.T.; Xie, J. Antimicrobial silver nanomaterials. Coord. Chem. Rev 2018, 357, 1-17. [CrossRef] open in new tab
- Muñoz, L.; Tamayo, L.; Gulppi, M.; Rabagliati, F.; Flores, M.; Urzúa, M.; Azócar, M.; Zagal, J.H.; Encinas, M.V.; Zhou, X.; et al. Surface functionalization of an aluminum alloy to generate an antibiofilm coating based on poly(methyl methacrylate) and silver nanoparticles. Molecules 2018, 23, 2747. [CrossRef] open in new tab
- Paiva, L.; Fidalgo, T.; Da Costa, L.; Maia, L.; Balan, L.; Anselme, K.; Ploux, L.; Thiré, R. Antibacterial properties and compressive strength of new one-step preparation silver nanoparticles in glass ionomer cements (NanoAg-GIC). J. Dent. 2018, 69, 102-109. [CrossRef] [PubMed] open in new tab
- Tamayo, L.; Azócar, M.; Kogan, M.; Riveros, A.; Páez, M. Copper-polymer nanocomposites: An excellent and cost-effective biocide for use on antibacterial surfaces. Mater. Sci. Eng. C 2016, 69, 1391-1409. [CrossRef] [PubMed] open in new tab
- Burdusel, A.C.; Gherasim, O.; Grumezescu, A.M.; Mogoanta, L.; Ficai, A.; Andronescu, E. Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials 2018, 8, 681. [CrossRef] [PubMed] open in new tab
- Das, B.; Dash, S.K.; Mandal, D.; Ghosh, T.; Chattopadhyay, S.; Tripathy, S.; Das, S.; Dey, S.K.; Das, D.; Roy, S. Green synthesized silver nanoparticles destroy multidrug resistant bacteria via reactive oxygen species mediated membrane damage. Arab. J. Chem. 2017, 10, 862-876. [CrossRef] open in new tab
- Akter, M.; Sikder, M.T.; Rahman, M.M.; Ullah, A.A.; Hossain, K.F.B.; Banik, S.; Hosokawa, T.; Saito, T.; Kurasaki, M. A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. J. Adv. Res. 2018, 9, 1-16. [CrossRef] [PubMed] open in new tab
- Graves, J.L., Jr.; Tajkarimi, M.; Cunningham, Q.; Campbell, A.; Nonga, H.; Harrison, S.H.; Barrick, J.E. Rapid evolution of silver nanoparticle resistance in Escherichia coli. Front. Genet. 2015, 6, 42. [CrossRef] [PubMed] open in new tab
- Graves, J.L., Jr. A Grain of Salt: Metallic and Metallic Oxide Nanoparticles as the New Antimicrobials. JSM Nanotechnol. Nanomed. 2014, 2, 1026.
- Bapat, R.A.; Joshi, C.P.; Bapat, P.; Chaubal, T.V.; Pandurangappa, R.; Jnanendrappa, N.; Gorain, B.; Khurana, S.; Kesharwani, P. The use of nanoparticles as biomaterials in dentistry. Drug Discov. Today 2019, 24, 85-98. [CrossRef] open in new tab
- Palza, H.; Escobar, B.; Bejarano, J.; Bravo, D.; Diaz-Dosque, M.; Pérez, J. Designing antimicrobial bioactive glass materials with embedded metal ions synthesized by the sol-gel method. Mater. Sci. Eng. C 2013, 33, 3795-3801. [CrossRef] open in new tab
- Chatterjee, A.K.; Chakraborty, R.; Basu, T. Mechanism of antibacterial activity of copper nanoparticles. Nanotechnology 2014, 25, 135101. [CrossRef] open in new tab
- Savelyev, Y.; Gonchar, A.; Movchan, B.; Gornostay, A.; Vozianov, S.; Rudenko, A.; Rozhnova, R.; Travinskaya, T. Antibacterial polyurethane materials with silver and copper nanoparticles. Mater. Today Proc. 2017, 4, 87-94. [CrossRef] open in new tab
- Bejarano, J.; Caviedes, P.; Palza, H. Sol-gel synthesis and in vitro bioactivity of copper and zinc-doped silicate bioactive glasses and glass-ceramics. Biomed. Mater. 2015, 10, 25001. [CrossRef] [PubMed] open in new tab
- Theodorou, G.S.; Kontonasaki, E.; Theocharidou, A.; Bakopoulou, A.; Bousnaki, M.; Hadjichristou, C.; Papachristou, E.; Papadopoulou, L.; Kantiranis, N.A.; Chrissafis, K.; et al. Sol-Gel Derived Mg-Based Ceramic Scaffolds Doped with Zinc or Copper Ions: Preliminary Results on Their Synthesis, Characterization, and Biocompatibility. Int. J. Biomater. 2016, 2016, 3858301. [CrossRef] [PubMed] open in new tab
- Gutierrez, M.F.; Alegria-Acevedo, L.F.; Mendez-Bauer, L.; Bermudez, J.; Davila-Sanchez, A.; Buvinic, S.; Hernandez-Moya, N.; Reis, A.; Loguercio, A.D.; Faragao, P.V.; et al. Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles. J. Dent. 2019, 82, 45-55. [CrossRef] open in new tab
- Banerjee, S.; Bagchi, B.; Bhandary, S.; Kool, A.; Hoque, N.A.; Thakur, P.; Das, S. A facile vacuum assisted synthesis of nanoparticle impregnated hydroxyapatite composites having excellent antimicrobial properties and biocompatibility. Ceram. Int. 2018, 44, 1066-1077. [CrossRef] open in new tab
- Shen, S.-C.; Letchmanan, K.; Chow, P.S.; Tan, R.B.H. Antibiotic elution and mechanical property of TiO 2 nanotubes functionalized PMMA-based bone cements. J. Mech. Behav. Biomed. Mater. 2019, 91, 91-98. [CrossRef] [PubMed] open in new tab
- Khandaker, M.; Li, Y.; Morris, T. Micro and nano MgO particles for the improvement of fracture toughness of bone-cement interfaces. J. Biomech. 2013, 46, 1035-1039. [CrossRef] open in new tab
- Khaled, S.; Charpentier, P.A.; Rizkalla, A.S. Synthesis and characterization of poly(methyl methacrylate)-based experimental bone cements reinforced with TiO 2 -SrO nanotubes. Acta Biomater. 2010, 6, 3178-3186. [CrossRef] open in new tab
- Kamonkhantikul, K.; Arksornnukit, M.; Takahashi, H. Antifungal, optical, and mechanical properties of polymethylmethacrylate material incorporated with silanized zinc oxide nanoparticles. Int. J. Nanomed. 2017, 12, 2353-2360. [CrossRef] open in new tab
- Cierech, M.; Osica, I.; Kolenda, A.; Wojnarowicz, J.; Szmigiel, D.; Łojkowiski, W.; Kurzydłowski, K.; Ariga, K.; Mierzwińska-Nastalska, E. Mechanical and Physicochemical Properties of Newly Formed ZnO-PMMA Nanocomposites for Denture Bases. Nanomaterials 2018, 8, 305. [CrossRef] open in new tab
- Russo, T.P.; Gloria, A.B.; De Santis, R.; D'Amora, U.; Barbaric, K.; Vollaro, A.; Oliviero, O.; Improta, G.; Triassi, M.; Ambrosio, L. Preliminary focus on the mechanical and antibacterial activity of a PMMA-based bone cement loaded with gold nanoparticles. Bioact. Mater. 2017, 2, 156-161. [CrossRef] [PubMed] open in new tab
- Totu, E.E.; Nechifor, A.C.; Nechifor, G.; Aboul-Enein, H.Y.; Cristache, C.M. Poly(methyl methacrylate) with TiO 2 nanoparticles inclusion for stereolitographic complete denture manufacturing-The future in dental care for eldery edentulous patients? J. Dent. 2017, 59, 68-77. [CrossRef] [PubMed] open in new tab
- Slane, J.; Vivanco, J.; Rose, W.; Ploeg, H.L.; Squire, M. Mechanical, material, and antimicrobial properties of acrylic bone cement impregnated with silver nanoparticles. Mater. Sci. Eng. C 2015, 48, 188-196. [CrossRef] [PubMed] open in new tab
- Lyutakov, O.; Goncharova, I.; Rimpelova, S.; Kolarova, K.; Svanda, J.; Svorcik, V. Silver release and antimicrobial properties of PMMA films doped with silver ions, nano-particles and complexes. Mater. Sci. Eng. C 2015, 49, 534-540. [CrossRef] [PubMed] open in new tab
- International Standard ISO 5833. Implants for Surgery-Acrylic Resin Cements; International Standard ISO: Geneva, Switzerland, 2002. open in new tab
- Wekwejt, M.; Moritz, M.;Świeczko-Żurek, B.; Pałubicka, A. Biomechanical testing of bioactive bone cements-A comparison of the impact of modifiers: Antibiotics and nanometals. Polym. Test. 2018, 70, 234-243. [CrossRef] open in new tab
- Wekwejt, M.; Pałubicka, A. Antibacterial evaluation of bioactive modifiers of bone cements: Antibiotics, nanometals and chitosan. Eur. J. Med. Technol. 2018, 3, 6-10. open in new tab
- Clinical & Laboratory Standards Institute. M07: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 11th ed.; Clinical & Laboratory Standards Institute: Wayne, PA, USA, 2018. 50. International Standard for Blood Banks & Blood Transfusion Services; NACO: New Delhi, India, 2007. open in new tab
- Henkelman, S.; Rakhorst, G.; Blanton, J.; Van Oeveren, W. Standardization of incubation conditions for hemolysis testing of biomaterials. Mater. Sci. Eng. C 2019, 29, 1650-1654. [CrossRef] open in new tab
- Wang, J.; Green, P.S.; Simpkins, J.W. Estradiol protects against ATP depletion, mitochondrial membrane potential decline and the generation of reactive oxygen species induced by 3-nitroproprionic acid in SK-N-SH human neuroblastoma cells. J. Neurochem. 2001, 77, 804-811. [CrossRef] open in new tab
- Ginebra, M.P.; Montufar, E.B. Cements as bone repair materials. In Bone Repair Biomaterials; open in new tab
- Pawelec, K.M., Planell, J.A., Eds.; Woodhead Publishing: Cambridge, UK, 2019; pp. 233-271.
- Potdar, P.D.; Jethmalani, Y.D. Human dental pulp stem cells: Applications in future regenerative medicine. World J. Stem Cells 2015, 7, 839-851. [CrossRef] open in new tab
- Nussler, A.; Sajadian, S.O. Adult Stem Cells. Stem Cell Rev. 2015, 1553, 3-23. open in new tab
- Fischer, A.H.; Jacobson, K.A.; Rose, J.; Zeller, R. Preparation of Cells and Tissues for Fluorescence Microscopy. In Basic Methods in Microscopy: Protocols and Concepts from Cells: A Laboratory Manual; open in new tab
- Spectro, D.L., Goldman, R.D., Eds.; Cold Spring Harbor Laboratory Press: Laurel Hollow, NY, USA, 2005.
- Parameswaran, S.; Verma, R.S. Scanning electron microscopy preparation protocol for differentiated stem cells. Anal. Biochem. 2011, 416, 186-190. [CrossRef] [PubMed] open in new tab
- Class II Special Controls Guidance Document: Polymethylmethacrylate (PMMA) Bone Cement-Guidance for Industry and FDA. Available online: https://fda.gov/regulatory-information/search-fda-guidance- documents/class-ii-special-controls-guidance-document-polymethylmethacrylate-pmma-bone-cement- guidance (accessed on 22 July 2019). open in new tab
- Bauer, S.; Schmuki, P.; von der Mark, K.; Park, J. Engineering biocompatible implant surfaces: Part I: Materials and surfaces. Prog. Mater. Sci. 2013, 58, 261-326. [CrossRef] open in new tab
- Martínez-Moreno, J.; Mura, C.; Merino, V.; Nácher, A.; Climente, M.; Merino-Sanjuán, M. Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin. J. Arthroplasty 2015, 30, 1243-1249. [CrossRef] open in new tab
- Rathbone, C.R.; Cross, J.D.; Brown, K.V.; Murray, C.K.; Wenke, J.C. Effect of various concentrations of antibiotics on osteogenic cell viability and activity. J. Orthop. Res. 2011, 29, 1070-1074. [CrossRef] [PubMed] open in new tab
- Prokopovich, P.; Köbrick, M.; Brousseau, E.; Perni, S. Potent antimicrobial activity of bone cement encapsulating silver nanoparticles capped with oleic acid. J. Biomed. Mater. Res. Part B Appl. Biomater. 2015, 103, 273-281. [CrossRef] [PubMed] open in new tab
- Prokopovich, P.; Leech, R.; Carmalt, C.J.; Parkin, I.P.; Persi, S. A novel bone cement impregnated with silver-tiopronin nanoparticles: Its antimicrobial, cytotoxic, and mechanical properties. Int. J. Nanomed. 2013, 8, 2227-2237. [CrossRef] [PubMed] open in new tab
- Alt, V.; Bechert, T.; Steinrücke, P.; Wagener, M.; Seidel, P.; Dingeldein, E.; Domann, E.; Schnettler, R. An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials 2004, 25, 4383-4391. [CrossRef] [PubMed] open in new tab
- Pauksch, L.; Hartmann, S.; Szalay, G.; Alt, V.; Lips, K.S. In vitro assessment of nanosilver-functionalized PMMA bone cement on primary human mesenchymal stem cells and osteoblasts. PLoS ONE 2014, 9, e114740. [CrossRef] open in new tab
- Choi, J.; Reipa, V.; Hitchins, V.M.; Goering, P.L.; Malinauskas, R.A. Physicochemical characterization and in vitro hemolysis evaluation of silver nanoparticles. Toxicol. Sci. 2011, 123, 133-143. [CrossRef] open in new tab
- Huang, H.; Lai, W.; Cui, M.; Liang, L.; Lin, Y.; Fang, Q.; Liu, Y.; Xie, L. An Evaluation of Blood Compatibility of Silver Nanoparticles. Sci. Rep. 2016, 6, 25518. [CrossRef] [PubMed] open in new tab
- Petrochenko, P.E.; Zheng, J.; Casey, B.J.; Bayati, M.R.; Narayan, R.J.; Goering, P.L. Nanosilver-PMMA composite coating optimized to provide robust antibacterial efficacy while minimizing human bone marrow stromal cell toxicity. Toxicol. Vitr. 2017, 44, 248-255. [CrossRef] [PubMed] open in new tab
- Chen, Z.; Meng, H.; Xing, G.; Chen, C.; Zhao, Y.; Zhu, C.; Fang, X.; Ma, B.; Wan, L. Acute toxicological effects of copper nanoparticles in vivo. Toxicol. Lett. 2006, 163, 109-120. [CrossRef] [PubMed] open in new tab
- Zhou, X.; Zhao, L.; Tang, H.; Xu, M.; Wang, Y.; Yang, X.; Chen, H.; Li, Y.; Ye, G.; Shi, F.; et al. The Toxic Effects and Mechanisms of Nano-Cu on the Spleen of Rats. Int. J. Mol. Sci. 2019, 20, 1469. [CrossRef] [PubMed] open in new tab
- Jaidev, L.R.; Kumar, S.; Chatterjee, K. Multi-biofunctional polymer graphene composite for bone tissue regeneration that elutes copper ions to impart angiogenic, osteogenic and bactericidal properties. Colloids Surf. B 2017, 159, 293-302. [CrossRef] [PubMed] open in new tab
- Hidalgo-Robatto, B.M.; López-Alvarez, M.; Azevedo, A.S.; Dorado, J.; Serra, J.; Azevedo, N.F.; Gonzalez, P. Pulsed laser deposition of copper and zinc doped hydroxyapatite coatings for biomedical applications. Surf. Coat. Technol. 2018, 333, 168-177. [CrossRef] open in new tab
- Hu, L.X.; Hu, S.F.; Rao, M.; Yang, J.; Lei, H.; Duan, Z.; Xia, W.; Zhu, C. Studies of acute and subchronic systemic toxicity associated with a copper/low-density polyethylene nanocomposite intrauterine device. Int. J. Nanomed. 2018, 13, 4913-4926. [CrossRef] open in new tab
- Wu, C.; Zhou, Y.; Xu, M.; Han, P.; Chen, L.; Chang, J.; Xiao, Y. Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity. Biomaterials 2013, 34, 422-433. [CrossRef] open in new tab
- Ryan, E.J.; Ryan, A.J.; González-Vázquez, A.; Philippart, A.; Ciraldo, F.E.; Hobbs, C.; Nicolosi, V.; Boccaccini, A.R.; Kearney, C.J.; O'Brien, F.J. Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo. Biomaterials 2019, 197, 405-416. [CrossRef] open in new tab
- Milkovic, L.; Hoppe, A.; Detsch, R.; Boccaccini, A.R.; Zarkovic, N. Effects of Cu-doped 45S5 bioactive glass on the lipid peroxidation-associated growth of human osteoblast-like cells in vitro. J. Biomed. Mater. Res. Part A 2014, 102, 3556-3561. [CrossRef] open in new tab
- Keller, A.A.; Adeleye, A.S.; Conway, J.R.; Garner, K.L.; Zhao, L.; Cherr, G.N.; Hong, J.; Gardea-Torresdey, J.L.; Godwin, H.A.; Hanna, S.; et al. Comparative environmental fate and toxicity of copper nanomaterials. NanoImpact 2017, 7, 28-40. [CrossRef] open in new tab
- Sawant, S.N.; Selvaraj, V.; Prabhawathi, V.; Doble, M. Antibiofilm Properties of Silver and Gold Incorporated PU, PCLm, PC and PMMA Nanocomposites under Two Shear Conditions. PLoS ONE 2013, 8, e63311. [CrossRef] [PubMed] open in new tab
- Moojen, D.J.F.; Vogely, H.C.; Fleer, A.; Verbout, A.J.; Castelein, R.M.; Dhert, W.J.A. No efficacy of silver bone cement in the prevention of methicillin-sensitive Staphylococcal infections in a rabbit contaminated implant bed model. J. Orthop. Res. 2009, 27, 1002-1007. [CrossRef] [PubMed] open in new tab
- Navarro-Rosales, M.; Ávila-Orta, C.A.; Neira-Velázquez, M.G.; Ortega-Ortiz, H.; Hernández-Hernández, E.; Solís-Rosales, S.G.; España-Sánchez, B.L.; Gõnzalez-Morones, P.; Jímenez-Barrera, R.M.; Sánchez-Valdes, S.; et al. Effect of plasma modification of copper nanoparticles on their antibacterial properties. Plasma Process Polym 2014, 11, 685-693. [CrossRef] open in new tab
- Anyaogu, K.C.; Fedorov, A.C.; Neckers, D.C. Synthesis, characterization, and antifouling potential of functionalized copper nanoparticles. Langmuir 2008, 24, 4340-4346. [CrossRef] [PubMed] open in new tab
- Balela, M.D.L.; Amores, K.L.S. Electroless deposition of copper nanoparticle for antimicrobial coating. Mater. Chem. Phys. 2019, 225, 393-398. [CrossRef] open in new tab
- Miola, M.; Cochis, A.; Kumar, A.; Arciola, C.R.; Rimondini, L.; Verné, E. Copper-doped bioactive glass as filler for PMMA-based bone cements: Morphological, mechanical, reactivity, and preliminary antibacterial characterization. Materials 2018, 11, 961. [CrossRef] open in new tab
- Khaaton, U.T.; Rao, N.G.V.S.; Mantravadi, K.M.; Ramanaviciene, A.; Ramanavivius, A. Antibacterial and antifungal activity of silver nanospheres synthesized by tri-sodium citrate assisted chemical approach. Vacuum 2017, 146, 259-265. [CrossRef] open in new tab
- Aleksandr, L.; Alexander, P.; Olga, B.; Sergey, K.; Irena, G. Synthesis of antimicrobial AlOOH-Ag composite nanostructures by water oxidation of bimetallic Al-Ag nanoparticles. RSC Adv. 2018, 8, 36239-36244. [CrossRef] open in new tab
- Kaygusuz, M.; Lkhagvajav, N.; Yasa, I.; Celik, E. Antimicrobial Nano-Ag-TiO 2 coating for lining leather. Rom. Biotechnol. Lett. 2016, 21, 18866-18874.
- Sopjani, M.; Föller, M.; Haendeler, J.; Götz, F.; Lang, F. Silver ion-induced suicidal erythrocyte death. J Appl Toxicol 2009, 29, 531-536. [CrossRef] open in new tab
- Pandey, R.K.; Prajapati, V.K. Molecular and immunological toxic effects of nanoparticles. Int. J. Biol. Macromol. 2017, 107, 1278-1293. [CrossRef] [PubMed] open in new tab
- Luo, Y.H.; Chang, L.W.; Lin, P. Metal-Based Nanoparticles and the Immune System: Activation, Inflammation, and Potential Applications. BioMed Res. Int. 2015, 2015, 143720. [CrossRef] [PubMed] open in new tab
- © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). open in new tab
- Verified by:
- Gdańsk University of Technology
seen 400 times