Biogenic amines content can be the source of information in the field of freshness and suitability of certain foods for consumption. However, most analytical methods for their determination require complicated sample preparation and expert knowledge. Because of that, a novel approach based on chromogenic dye application was proposed. After the SN1 reaction of an amine with S 0378 dye, the absorbance of the solution was measured using spectrophotometry. With the proposed methodology, it was possible to determine total biogenic amines content in the range of 0.5–100 μg L–1 with a determination coefficient exceeding 0.98.
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