Search
Abstract
The aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganism to the expression vectors in the Tabor-Studier system and its expression in Rosetta(DE3)pLysS Escherichia coli cells. The second phase of the study consisted of proteins purification using an initial denaturation of host proteins and salting-out proteins by ammonium sulfate.
Authors (3)
Cite as
Full text
full text is not available in portal
Keywords
Details
- Category:
- Other
- Type:
- supllement, wydanie specjalne, dodatek
- Published in:
-
Acta Biochimica Polonica
no. 59,
ISSN: 0001-527X - Title of issue:
- Acta Biochimica Polonica.
- Language:
- English
- Publication year:
- 2012
- Bibliographic description:
- Panek A., Filipkowski P., Synowiecki J.:Cloning, Expression and Purification of Wild-Type Trehalose Synthase from Deinococcus geothermalis//.-Vol. 59,nr. suppl. 3(2012),
- Verified by:
- Gdańsk University of Technology
seen 159 times