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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
Abstract
A new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific linker. Only DNA fragmens which consist pehX gene are amplified from the ligation product by PCR. Variety of PCR product length can differentiate the K. oxytoca strains among species.
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- Category:
- Other
- Type:
- supllement, wydanie specjalne, dodatek
- Published in:
-
Acta Biochimica Polonica
no. 58,
ISSN: 0001-527X - Title of issue:
- Acta Biochimica Polonica.
- Language:
- English
- Publication year:
- 2011
- Bibliographic description:
- Stojowska K., Krawczyk B., Kur J.: DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation// Acta Biochimica Polonica. -Vol. 58., nr. suppl. 4 (2011),
- Verified by:
- Gdańsk University of Technology
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