Dehydration converts DsbG crystal diffraction from low to high resolution

Begoña Heras; Melissa A. Edeling; Karl A. Byriel; Alun Jones; Satish Raina; Jennifer L. Martin

doi:10.1016/s0969-2126(03)00005-4

Dehydration converts DsbG crystal diffraction from low to high resolution

Abstract

Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 Å resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.

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Authors (6)

Begoña Heras
Melissa A. Edeling
Karl A. Byriel
Alun Jones
Satish Raina prof. dr
Jennifer L. Martin

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DOI:: Digital Object Identifier (open in new tab) 10.1016/S0969-2126(03)00005-4
License: Copyright (2003 Elsevier Science Ltd.)

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Details

Category:: Articles
Type:: artykuły w czasopismach
Published in:: STRUCTURE no. 11, pages 139 - 145,
ISSN: 0969-2126
Language:: English
Publication year:: 2003
Bibliographic description:: Heras B., Edeling M. A., Byriel K. A., Jones A., Raina S., Martin J. L.: Dehydration converts DsbG crystal diffraction from low to high resolution// STRUCTURE -Vol. 11,iss. 2 (2003), s.139-145
DOI:: Digital Object Identifier (open in new tab) 10.1016/s0969-2126(03)00005-4
Verified by:: Gdańsk University of Technology