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Effects of unsymmetrical bisacridines on the spherical culture system of HCT116 colon and H460 lung cancer cell lines.
Abstract
3D-spheroid cultures are receiving increasing attention in cancer research, as they serve as a link between simplified 2D monolayer culture structure and the highly complex nature of tumors in vivo. Therefore, spherical cultures are a good model for the effective testing of anticancer drugs. Unsymmetrical bisacridines (UAs) are new antitumor compounds, patented in Europe and in the USA. Here, two cancer cell lines: colon HCT116 and lung H460, selected depending on their high sensitivity to UAs, were tested for their ability to form spheroids. Furthermore, the effects of four UAs: C-2028, C-2041, C 2045, C-2053 on the morphology, size and viability of HCT116 and H460 spheroids were investigated. We showed that both studied cell lines formed spheroids with different morphometric features. The HCT116-spheres were more condensed and had smooth, even periphery. The H460-spheres were bigger and presented more irregular shape. Treatment of 3D-spheroid cultures with UAs resulted in significant inhibition of their growth, however to a much lesser extent by C 2041 than others. Cell viability in spheroids exposed to UAs for 7 days was evaluated using the 7 AAD dye. Both HCT116- and H460-spheres presented significant fraction of non-viable cells reaching even 98% of the cellular population. The percentage of 7-AAD+ cells in 2D cultures after 72-hour incubation with UAs reached 29 to 54% in HCT116 cells and 78 to 93% in H460 cells. Differences in the ratio of live/dead cells in 2D and 3D cultures may result from distinct penetration of UAs through spherical cultures than in monolayer. Some compounds require prolonged exposure times to observe an effect on cell viability in vitro. Therefore, 3D spheroids cultures appear to better reflect the in vivo tumor growth than adherent model and are a good tool for evaluation of antitumor properties of our promising bisacridines compounds. These studies were supported by the National Science Center, Poland, No. UMO 2016/23/B/NZ7/03324.
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Keywords
Details
- Category:
- Articles
- Type:
- suplement w czasopiśmie
- Published in:
-
FEBS Open Bio
no. 11,
ISSN: 2211-5463 - Language:
- English
- Publication year:
- 2021
- Bibliographic description:
- Kulesza J., Pawłowska M., Augustin E.: Effects of unsymmetrical bisacridines on the spherical culture system of HCT116 colon and H460 lung cancer cell lines.// / : , 2021,
- DOI:
- Digital Object Identifier (open in new tab) 10.1002/2211-5463.13205
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
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