Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage - Publication - Bridge of Knowledge

Search

Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage

Abstract

Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytesafter hydrogen peroxide (H2O2) treatment (250 lM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 lg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H2O2 and AgNP treatments.

Citations

  • 2 5

    CrossRef

  • 0

    Web of Science

  • 3 1

    Scopus

Cite as

Full text

download paper
downloaded 149 times
Publication version
Accepted or Published Version
License
Creative Commons: CC-BY-NC-ND open in new tab

Keywords

Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
FOOD AND CHEMICAL TOXICOLOGY no. 50, pages 3352 - 3359,
ISSN: 0278-6915
Publication year:
2012
Bibliographic description:
Hudecova A., Kusznierewicz B., Hasplova K., Huk A., Magdolenova Z., Miadokova E., Galova E., Dusinska M.: Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage// FOOD AND CHEMICAL TOXICOLOGY. -Vol. 50, nr. iss. 9 (2012), s.3352-3359
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.fct.2012.06.017
Bibliography: test
  1. Ahamed, M., Posgai, R., Gorey, T.M., Nielsen, M., Hussain, S.M., Rowe, J.J., 2010. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol. Appl. Pharmacol. 242, 263-269. open in new tab
  2. Aherne, S.A., Kerry, J.P., Ó Brien, N.M., 2007. Effects of plant extracts on antioxidant status and oxidant-induced stress in Caco-2 cells. Br. J. Nutr. 97, 321-328. open in new tab
  3. Azqueta A., Shaposhnikov, S., Collins, A.R., 2011. DNA Repair Measured by the comet assay, DNA Repair, Dr. Inna Kruman (Ed.), ISBN: 978-953-307-697-3, InTech. open in new tab
  4. Baute, J., Depicker, A., 2008. Base excision repair and its role in maintaining genome stability. Crit. Rev. Biochem. Mol. Biol. 43, 239-276. open in new tab
  5. Collins, A.R., Azqueta, A., Langie, S.A., 2012. Effects of micronutrients on DNA repair. Eur. J. Nutr. 51, 261-279. open in new tab
  6. Cheng, D., Yang, J., Zhao, Y., 2004. Antibacterial materials of silver nanoparticles application in medical appliances and appliances for daily use. Chin. Med. Equip. J. 4, 26-32. open in new tab
  7. Dar, A., Faizi, S., Naqvi, S., Roome, T., Zikr-Ur-Rehman, S., Ali, M., Firdous, S., Moin, S.T., 2005. Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biol. Pharm. Bull. 28, 596-600. open in new tab
  8. Das, S., Nageshwar, R.B., Satish, R.B.S., 2011. Mangiferin attenuates methylmercury induced cytotoxicity against IMR-32, human neuroblastoma cells by the inhibition of oxidative stress and free radical scavenging potential. Chem. Biol. Interact. 193, 129-140. open in new tab
  9. Decordier, I., Loock, K.V., Kirsch-Volders, M., 2010. Phenotyping for DNA repair capacity. Mutat. Res. 705, 107-129. open in new tab
  10. Dianov, G.L., Parsons, J.L., 2007. Co-ordination of DNA single strand break repair. DNA Repair (Amst.) 6, 454-460. open in new tab
  11. Dudonné, S., Vitrac, X., Coutière, P., Woillez, M., Mérillon, J.M., 2009. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J. Agric. Food Chem. 57, 1768-1774. open in new tab
  12. Dusinska M, Staruchova M, Horska A, Smolkova B, Collins A, Bonassi S, Volkovova K. Are glutathione S transferases involved in DNA damage signalling? 2012. Interactions with DNA damage and repair revealed from molecular epidemiology studies. Mutat Res. Mar 20. [Epub ahead of print]. open in new tab
  13. Finnegan, M., Linley, E., Denyer, S.P., McDonnell, G., Simons, C., Maillard, J.Y., 2010. Mode of action of hydrogen peroxide and other oxidizing agents: differences between liquid and gas forms. J. Antimicrob. Chemother. 65, 2108-2115. open in new tab
  14. Friedberg, E.C., 1985. Nucleotide excision repair of DNA in eukaryotes: comparisons between human cells and yeast. Cancer Surv. 4, 529-555. open in new tab
  15. Halamoda Kenzaoui, B., Chapuis Bernasconi, C., Guney-Ayra, S., Juillerat-Jeanneret, L., 2012. Induction of oxidative stress, lysosome activation and autophagy by nanoparticles in human brain-derived endothelial cells. Biochem. J. 441, 813- 821. open in new tab
  16. Heo, S.K., Yi, H.S., Yun, H.J., Ko, C.H., Choi, J.W., Park, S.D., 2010. Ethylacetate extract from Draconis Resina inhibits LPS-induced inflammatory responses in vascular smooth muscle cells and macrophages via suppression of ROS production. Food Chem. Toxicol. 48, 1129-1136. open in new tab
  17. Hudecova, A., Hasplova, K., Miadokova, E., Magdolenova, Z., Rinna, A., Galova, E., Sevcovicova, A., Vaculcikova, D., Gregan, F., Dusinska, M., 2010. Cytotoxic and genotoxic effect of methanolic flower extract from Gentiana asclepiadea on Cos1 cells. Neuroendocrinol. Lett. 31, 21-25.
  18. Hudecova, A., Hasplova, K., Miadokova, E., Magdolenova, Z., Rinna, A., Collins, A.R., Galova, E., Vaculcikova, D., Gregan, F., Dusinska, M., 2012a. Gentiana asclepiadea protects human cells against oxidation DNA lesions. Cell Biochem. Funct. 30, 101-107. open in new tab
  19. Hudecova, A., Hasplova, K., Kellovska, L., Ikreniova, M., Miadokova, E., Galova, E., Horvathova, E., Vaculcikova, D., Gregan, F., Dusinska, M., 2012b. Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes. Neoplasma 59, 62-69. open in new tab
  20. Hudecova, A., Hasplova, K., Miadokova, E., Galova, E., Dusinska, M., 2012c. Comparison of potential cytotoxicity and genotoxicity of 20 and 200 nm silver nanoparticles on HEK 293 cells after 30 min and 24 h exposure, Students Scientific Conference, 25.4.2012, Bratislava, Slovakia, ISBN 978-80-223-3213-2.
  21. Hudecova, A., Magdolenova, Z., Kusznierewicz, B., Hasplova, K., Rinna, A., Runden- Pran, E., Fjellsbø, L.M., Kruszewski, M., Lankoff, A., Sandberg, W.J., Refsnes, M., Skuland, T., Schwarze, P., Brunborg, G., Bjøras, M., Collins, A., Miadokova, E., Galova, E., Dusinska, M, (2012d). Silver nanoparticles induce pre-mutagenic DNA base lesions that can be prevented by treatment with plant extracts from Gentiana asclepiadea. Mutagenesis, accepted for publication. open in new tab
  22. Jain, P., Pradeep, T., 2005. Potential of silver nanoparticle-coated polyurethane foam as an antibacterial water filter. Biotechnol. Bioeng. 90, 59-63. open in new tab
  23. Jaishree, V., Badami, S., 2010. Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against D-galactosamine induced acute liver damage in rats. J. Ethnopharmacol. 130, 103-106. open in new tab
  24. Jensen, S.R., Schripsema, J., 2002. Chemotaxonomy and Pharmacology of Gentianaceae. In: Struwe, L., Albert, V. (Eds.), Gentianaceae -Systematic and Natural History. Cambridge University Press, pp. 573-631. open in new tab
  25. Kang, J., Li, Z., Wu, T., Jensen, G.S., Schauss, A.G., Wu, X., 2010. Anti-oxidant capacities of flavonoid compounds isolated from acai pulp (Euterpe oleracea Mart.). Food Chem. 122, 610-617. open in new tab
  26. Kovacs, E., 2002. The in vitro effect of Viscum album (VA) extract on DNA repair of peripheral blood mononuclear cells (PBMC) in cancer patients. Phytother. Res. 16, 143-147. open in new tab
  27. Kusznierewicz, B., Piasek, A., Bartoszek, A., Namieśnik, J., 2011. The optimisation of analytical parameters for routine profiling of antioxidants in complex mixtures by HPLC coupled post-column derivatisation. Phytochem. Anal. 22, 392-402. open in new tab
  28. Laborde, E., 2010. Glutathione transferases as mediators of signalling pathways involved in cell proliferation and cell death. Cell Death Differ. 17, 1373-1380. open in new tab
  29. Martínez-Cayuela, M., 1995. Oxygen free radicals and human disease. Biochimie 77, 147-161. open in new tab
  30. Nić iforović , N., Mihailović , V., Masković , P., Solujić , S., Stojković , A., Pavlović Muratspahić , D., 2010. Antioxidant activity of selected plant species; potential new sources of natural antioxidants. Food Chem. Toxicol. 48, 3125-3130. open in new tab
  31. Mihailović , V., Vuković , N., Nić iforović , N., Solujić , S., Mladenović , M., Mašković , P., Stanković , M.S., 2011. Studies on the antimicrobial activity and chemical composition of the essential oils and alcoholic extracts of Gentiana asclepiadea L. J. Med. Plants Res. 5, 1164-1174. open in new tab
  32. Park, E.J., Yi, J., Kim, Y., Choi, K., Park, K., 2010. Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. Toxicol. In Vitro 24, 872- 878. open in new tab
  33. Piao, M.J., Kang, K.A., Lee, I.K., Kim, H.S., Kim, S., Choi, J.Y., Choi, J., Hyun, J.W., 2011. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol. Lett. 201, 92-100. open in new tab
  34. Ramos, A.A., Azqueta, A., Pereira-Wilson C., Collins, A.R., 2010. Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cells. J. Agric. Food. Chem. 58, 7465- 7471. open in new tab
  35. Rao, B.S.S., Sreedevi, M.V., Nageshwar Rao, B., 2009. Cytoprotective and antigenotoxic potential of Mangiferin, a glucosylxanthone against cadmium chloride induced toxicity in HepG2 cells. Food Chem. Toxicol. 47, 592-600.
  36. Saric, M., 1989. Medicinal Plants of SR Serbia, SASA, Belgrade, 9. 278 (in Serbian).
  37. Skandrani, I., Limem, I., Neffati, A., Boubaker, J., Ben Sghaier, M., Bhouri, W., Bouhlel, I., Kilani, S., Ghedira, K., Chekir-Ghedira, L., 2010a. Assessment of phenolic content, free-radical-scavenging capacity genotoxic and anti-genotoxic effect of aqueous extract prepared from Moricandia arvensis leaves. Food Chem. Toxicol. 48, 710-715. open in new tab
  38. Skandrani, I., Boubaker, J., Bhouri, W., Limem, I., Kilani, S., Ben Sghaier, M., Neffati, A., Bouhlel, I., Ghedira, K., Chekir-Ghedira, L., 2010b. Leaf extracts from Moricandia arvensis promote antiproliferation of human cancer cells, induce apoptosis, and enhance antioxidant activity. Drug Chem. Toxicol. 33, 20-27. open in new tab
  39. Sevcovicova, A., Hercegova, A., 2010. Recent advances on DNA Damage, its Repair and Genomic Stability of Green Algae Chlamydomonas reinhardtii and other Photoautotrophic Eukaryotes. In: DNA Repair: Damage, Repair Mechanisms and Aging, ed. Frank Columbus, Nova Publishers, ISBN: 978-1-61668-914-8.
  40. Szucs, Z., Danos, B., Nyiredy, S., 2002. Comparative analysis of the underground parts of Gentiana species by HPLC with diode-array and mass spectrometric detection. Chromatographia (Suppl. 56), 19-23. open in new tab
  41. Wauthoz, N., Balde, A., Balde, E.S., Van Damme, M., Duez, P., 2007. Ethnopharmacology of Mangifera indica L. bark and pharmacological studies of its main C-glucosylxanthone, mangiferin. Int. J. Biomed. Pharmaceut. Sci. 1, 112-119.
  42. Zeraik, M.L., Serteyn, D., Deby-Dupont, G., Wauters, J.N., Tits, M., Yariwake, J.H., Angenot, L., Franck, T., 2011. Evaluation of the antioxidant activity of passion fruit (Passiflora edulis and Passiflora alata) extracts on stimulated neutrophils and myeloperoxidase activity assays. Food Chem. 128, 259-265. open in new tab
  43. Zhang, Y., Sun, J., 2007. A study on the bio-safety for nano-silver as anti-bacterial materials. Chin. J. Med. Instrum. 31, 35-38.
  44. Zheng, P., Zhang, K., Wang, Z., 2011. Genetic diversity and gentiopicroside content of four Gentiana species in China revealed by ISSR and HPLC methods. Biochem. Syst. Ecol. 39, 704-710. open in new tab
  45. Zieliń ska, D., Zieliń ski, H., 2011. Antioxidant activity of flavone C-glucosides determined by updated analytical strategies. Food Chem. 124, 672-678. open in new tab
Verified by:
Gdańsk University of Technology

seen 117 times

Recommended for you

Meta Tags