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Glucose oxidase as an important yet overlooked factor determiningthe antibacterial activity of bee pollen and bee bread
Abstract
Bee pollen (BP) and bee bread (BB) have attracted great attention due to their biological activities including antibacterial activity. However, the mechanism of antibacterial activity is largely unknown. Therefore, we aimed to characterise the antibacterial effect of BP and BB aqueous extracts against bacterial pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and Enterococcus faecalis) and identify the key compound(s) responsible for this effect. Here, we demonstrate that BP and particularly BB extracts display antibacterial activity which is significantly increased in the presence of glucose. Immunoblot analysis of extracts revealed the presence of MRJP1 in all analysed BP and BB samples and the enzyme glucose oxidase (GOX) in the majority of BB samples. Treatment of extracts with catalase resulted in the restoration of bacterial growth but only in those samples where glucose supplementation caused the enhancement of antibacterial activity. Our findings provide a deeper understanding of antibacterial activity of BP/BB which is mediated by the enzymatic activity of bee-derived GOX.
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Authors (5)
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Marcela Bucekova
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Jana Godocikova
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Juraj Majtan
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
no. 248,
pages 2929 - 2939,
ISSN: 1438-2377 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Pełka K., Bucekova M., Godocikova J., Szweda P., Majtan J.: Glucose oxidase as an important yet overlooked factor determiningthe antibacterial activity of bee pollen and bee bread// EUROPEAN FOOD RESEARCH AND TECHNOLOGY -Vol. 248, (2022), s.2929-2939
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00217-022-04101-z
- Sources of funding:
-
- Project Assess the potential of bee products and bacteriocins synthesized by the microflora of bee products as an alternative or complement to classical antibiotics
- Zintegrowany Program Rozwoju Politechniki Gdańskiej POWR.03.05.00-00-Z044/17
- Verified by:
- Gdańsk University of Technology
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