Human serum interactions with phenolic and aroma substances of Kaffir(Citrus hystrix) and Key lime (Citrus aurantifolia) juices
Abstract
To understand the therapeutic application of polyphenols extracted from Kaffir (PolKaf) and Key (PolKey) limesdifferent analytical methods were applied. Based on quantitative analysis by two dimensional gas chromato-graphy (GC×GC) and time of flight mass spectrometry (TOFMS) it can be observed that the biggest differencesin the contents of selected terpenes of Kaffir and Key limes occur in chemical compounds such as limonene, citraland terpinen-4-ol. Limonene concentration is almost 5 times higher in the volatile fraction of Key lime than inKaffir lime. In the case of citral, the difference in concentration of this compound in Kaffir is 20 µg/g lower thanin Key lime. Higher concentration of terpinen-4-ol was noted in Kaffir lime samples and the content was almost23 times higher. Terpinen-4-ol is the major chemical compound of volatile fraction of Kaffir lime. Among thedetermined terpenes, potential markers of aroma were selected: terpinen-4-ol and citral for characterization ofKaffir and Key limes. Antioxidant assays revealed the highest bioactivity of Kaffir lime. Fluorescence studiesbetween the interaction of polyphenols with human serum albumin (HSA) showed relatively high bindingabilities in comparison with some antiplatelet drugs. The docking results showed that the hydrophobic residuesare responsible for the interaction with the phyto-constituents. Citral is the best scored ADMET descriptor. Theantioxidant strong affinity to HSA and synergism in bioactivity are the main indices in health application ofcitrus fruits.
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF LUMINESCENCE
no. 201,
pages 115 - 122,
ISSN: 0022-2313 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Shafreen R., Lubinska-Szczygeł M., Różańska A., Dymerski T., Namieśnik J., Katrich E., Gorinstein S.: Human serum interactions with phenolic and aroma substances of Kaffir(Citrus hystrix) and Key lime (Citrus aurantifolia) juices// JOURNAL OF LUMINESCENCE. -Vol. 201, (2018), s.115-122
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.jlumin.2018.04.010
- Verified by:
- Gdańsk University of Technology
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