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Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes

Abstract

It is still under investigation, whether all Borrelia sp. causing Lyme borreliosis and other diseases are already identified and properly classified as human pathogens. For this reason, it is of great importance to develop a diagnostic ELISA test that detects all Borrelia sp. The aim of this study was to identify conserved DNA and protein regions present in all currently known Borrelia sp. In experimental studies 31 available Borrelia sp. genomes were aligned and screened for the presence of evolutionary conserved regions. As a result of bioinformatics analysis, one evolutionally conserved DNA region encoding a core fragment of the S21 ribosomal protein was identified. Both a couple of genus-specific PCR primers and the S21 protein B-cell epitope were designed for prospective diagnostic purposes.

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Details

Category:
Magazine publication
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
Acta Biochimica Polonica no. 66, pages 1 - 4,
ISSN: 0001-527X
Language:
English
Publication year:
2019
Bibliographic description:
Kotłowski R., Holec-Gąsior L.: Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes// Acta Biochimica Polonica. -Vol. 66, (2019), s.1-4
DOI:
Digital Object Identifier (open in new tab) 10.18388/abp.2018_2657

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