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Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes
Abstract
Infection with the intracellular protozoan parasite Toxo¬plasma gondii (T. gondii) causes health problems to both humans and livestock and has a large economic impact worldwide. The immune response in sheep following infection with T. gondii was evaluated using six different combinations of plasmid DNA, recombinant antigen and adjuvant. Sheep were generally vaccinated twice by intramuscular injection with plasmid DNA containing gene sequences for either the surface antigen (SAG!) or the rhoptry protein (ROP1) of T. gondii. Two of the groups injected with plasmid DNA SAG1 were boosted with recombinant protein (SAG!). We investigated the efficacy of including oligodeoxynucleotides (ODNJ that contain CG motifs (CpG) and the gene coding for ovine granulocyte-macrophage colony stimulating factor (GM-CSF) as potential adjuvants. Administration of the plasmid encoding the ROP1 gene significantly enhanced both IFN-y production from peripheral blood cells when cul¬tured in vitro with Toxoplasma antigen, and ROPl-specific IgGl and lgG2 antibody levels present in serum. However, injection with SAG1 did not stimulate IFN-y production. These results indicate the potential of ROP1, given as plas¬mid DNA, as a potential vaccine candidate to protect sheep against T. gondii infection.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
PARASITE IMMUNOLOGY
no. 32,
pages 671 - 683,
ISSN: 0141-9838 - Language:
- English
- Publication year:
- 2010
- Bibliographic description:
- Li B., Olędzka G., Mcfarlane R., Spellerberg M., Smith S., Gelder F., Kur J., Stankiewicz M.: Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes// PARASITE IMMUNOLOGY. -Vol. 32, nr. iss. 9-10 (2010), s.671-683
- DOI:
- Digital Object Identifier (open in new tab) 10.1111/j.1365-3024.2010.01228.x
- Verified by:
- Gdańsk University of Technology
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