Integration of protein tethering in a rapid and label-free SERS screening platform for drugs of abuse

Soumik Siddhanta; Maciej Wróbel; Ishan Barman

doi:10.1039/c6cc00518g

Integration of protein tethering in a rapid and label-free SERS screening platform for drugs of abuse

Abstract

Surface enhanced Raman spectroscopy (SERS) has emerged as a promising technique for the rapid and ultrasensitive detection of molecular species such as drugs of abuse in biofluids. Yet, it remains a significant challenge to create a viable screening tool for multiple drug classes, owing to the lack of affinity of certain species for the SERS substrate and to the matrix interference in complex media. Here we report a protein tethering SERS approach, which blends plasmonic enhancement with facile drug binding, to engineer a rapid, label-free and versatile screening tool for narcotics. By exploiting the known binding attributes of human serum albumin, we determine the effective concentration of narcotics present in solution through differential enhancement of the spectral markers. In conjunction with chemometric methods, this approach not only enables unambiguous recognition of different drug classes, such as barbiturates, opiates, amphetamines and benzodiazepines, but also offers a lower limit of detection in comparison to direct SERS application. Through molecular docking simulations, we probe the mechanistic underpinnings of the protein tethering approach paving the way for narcotic detection in clinical samples in the near future.

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Authors (3)

Soumik Siddhanta
- Johns Hopkins University Mechanical Engineering
Maciej Wróbel dr inż.
Ishan Barman
- Johns Hopkins University Mechanical Engineering

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: CHEMICAL COMMUNICATIONS no. 52, edition 58, pages 9016 - 9019,
ISSN: 1359-7345
Language:: English
Publication year:: 2016
Bibliographic description:: Siddhanta S., Wróbel M., Barman I.: Integration of protein tethering in a rapid and label-free SERS screening platform for drugs of abuse// CHEMICAL COMMUNICATIONS. -Vol. 52, iss. 58 (2016), s.9016-9019
DOI:: Digital Object Identifier (open in new tab) 10.1039/c6cc00518g
Verified by:: Gdańsk University of Technology