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Measuring the density of DNA films using ultraviolet-visible interferometry

Abstract

In order to determine a proper value for the density of dry DNA films we have used a method based upon the measurement of interference effects in transmission spectra of thin DNA layers. Our results show that the methodology is effective and the density of DNA in this state, 1.407 g/cm3, is much lower than the commonly used 1.7 g/cm3. Obtaining accurate values for the DNA film density will allow the optical constants for DNA to be recalculated, which were previously obtained assuming a higher DNA density. Furthermore, since our recent investigations have shown a strong dependence of the sample composition on DNA film formation and thus on its density, such a method will be important in characterizing particle interactions with DNA film and their dose dependence.

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DOI:
Digital Object Identifier (open in new tab) 10.1103/PhysRevE.87.060701
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Copyright (2013 American Physical Society)

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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
PHYSICAL REVIEW E no. 87, edition 6, pages 1 - 4,
ISSN: 1539-3755
Language:
English
Publication year:
2013
Bibliographic description:
Śmiałek-Telega M., Jones N., Vronning Hoffmann S., Mason N.: Measuring the density of DNA films using ultraviolet-visible interferometry// PHYSICAL REVIEW E. -Vol. 87, iss. 6 (2013), s.1-4
DOI:
Digital Object Identifier (open in new tab) 10.1103/physreve.87.060701
Verified by:
Gdańsk University of Technology

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