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Modified DNA polymerases for PCR troubleshooting

Abstract

PCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase used, is shown. In addition, the reasons that might justify the need for modification of DNA polymerases, type of modifications, and links between modified DNA polymerases and PCR efficiency are described.

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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
JOURNAL OF APPLIED GENETICS no. 58, pages 133 - 142,
ISSN: 1234-1983
Language:
English
Publication year:
2017
Bibliographic description:
Śpibida M., Krawczyk B., Olszewski M., Kur J.: Modified DNA polymerases for PCR troubleshooting// JOURNAL OF APPLIED GENETICS. -Vol. 58, nr. 1 (2017), s.133-142
DOI:
Digital Object Identifier (open in new tab) 10.1007/s13353-016-0371-4
Verified by:
Gdańsk University of Technology

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