Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli

Masayuki Murata; Hiroko Fujimoto; Kaori Nishimura; Kannikar Charoensuk; Hiroshi Nagamitsu; Satish Raina; Tomoyuki Kosaka; Taku Oshima; Naotake Ogasawara; Mamoru Yamada

doi:10.1371/journal.pone.0020063

Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli

Abstract

The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with a single-gene knockout library provided a list of genes indispensable for growth at 47°C, called thermotolerant genes. Genes for which expression was affected by exposure to CHT were identified by DNA chip analysis. Unexpectedly, the former contents did not overlap with the latter except for dnaJ and dnaK, indicating that a specific set of non-heat shock genes is required for the organism to survive under such a severe condition. More than half of the mutants of the thermotolerant genes were found to be sensitive to H2O2 at 30°C, suggesting that the mechanism of thermotolerance partially overlaps with that of oxidative stress resistance. Their encoded enzymes or proteins are related to outer membrane organization, DNA double-strand break repair, tRNA modification, protein quality control, translation control or cell division. DNA chip analyses of essential genes suggest that many of the genes encoding ribosomal proteins are down-regulated at CHT. Bioinformatics analysis and comparison with the genomic information of other microbes suggest that E. coli possesses several systems for survival at CHT. This analysis allows us to speculate that a lipopolysaccharide biosynthesis system for outer membrane organization and a sulfur-relay system for tRNA modification have been acquired by horizontal gene transfer.

Citations

6 8

CrossRef
0

Web of Science
6 5

Scopus

Authors (10)

Cite as

Full text

download paper

downloaded 21 times

Publication version: Accepted or Published Version
License: open in new tab

full content of the article see on external site open in new tab

Keywords

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: PLOS ONE no. 6,
ISSN: 1932-6203
Language:: English
Publication year:: 2011
Bibliographic description:: Murata M., Fujimoto H., Nishimura K., Charoensuk K., Nagamitsu H., Raina S., Kosaka T., Oshima T., Ogasawara N., Yamada M.: Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli// PLOS ONE. -Vol. 6, iss. 6 (2011), s.e20063-
DOI:: Digital Object Identifier (open in new tab) 10.1371/journal.pone.0020063
Verified by:: Gdańsk University of Technology