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Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine
Abstract
Urinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein composition using fluorescence measurements. However, despite much interest in the clinical application of uEVs, their analysis using the NTA method is poorly described and requires meticulous sample preparation, experimental adjustment of instrument settings, and above all, an understanding of the limitations of the method. In the present work, we demonstrate the usefulness of an NTA. We also present problems encountered during analysis with possible solutions: the choice of sample dilution, the method of the presentation and comparison of results, photobleaching, and the adjustment of instrument settings for a specific analysis. We show that the NTA method appears to be a promising method for the determination of uEVs. However, it is important to be aware of potential problems that may affect the results.
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Authors (9)
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Kornelia Sałaga-zaleska
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Agnieszka Kuchta
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Beata Bzoma
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Gabriela Chyła-Danił
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Anna Safianowska
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Agata Płoska
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Alicja Dębska-ślizień
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Maciej Jankowski
Cite as
Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/ijms241512228
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- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
no. 24,
ISSN: 1661-6596 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Sałaga-Zaleska K., Kuchta A., Bzoma B., Chyła-Danił G., Safianowska A., Płoska A., Kalinowski L., Dębska-Ślizień A., Jankowski M.: Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine// INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -Vol. 24,iss. 15 (2023), s.12228-
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/ijms241512228
- Sources of funding:
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- Financing: POWR.03.02.00-00-I014/17-00, POWR.03.02.00-00-I014/17-00 and by the Ministry of Education and Science (grant no. 10/E-389/SPUB/SP /2020) . Also NCN or Poland (grant no. 2021/41/N/NZ5/01076 to K.S.-Z.).
- Verified by:
- Gdańsk University of Technology
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