Optimal conditions of naphthoquinones separation from carnivorous plants extracts using thin-layer chromatography and high performance liquid chromatography

Mariusz Jaszczołt; Anita Skrzypczak; Aleksandra Królicka; Lewandowski Aleksander; Marian Antoni Kamiński

doi:10.1055/s-0031-1282249

Optimal conditions of naphthoquinones separation from carnivorous plants extracts using thin-layer chromatography and high performance liquid chromatography

Abstract

Droseraceae plants are owned by the family of carnivorous plants, which in an unusual way adapt to environments poor in nutrients. Droseraceae obtain essential nutrients catching insects and other small invertebrantes. Plumbagin, chloroplumbagin, ramentaceon and droseron are naphthoquinones, which could be found in leaves and shoots of plants from Droseraceae family. In the literature there are a lot of information about the pharmacological properities of these secondary metabolites.The purpose of this research was to develop optimal conditions for separation of naphthoquinones contained in extracts of insectivorous plants of the species Dionaea muscipula Ellis, Drosera aliciae Raym.-Hamet, Drosera capensis L. and Drosera binata Labill.The experimental studies have been divided into two stages, the first included study using thin layer chromatography (TLC) and the second using high performance liquid chromatography (HPLC) technique. Both the studies by thin layer chromatography and high performance liquid chromatography was performed in normal and reversed phase system. Optimal conditions of naphthoquinones separation using TLC and HPLC in normal and reversed phase system will be presented.Acknowledgement: State Commitee for Scientific Research, Grant No N N405 3757 37. This research work was supported by the European Union in the framework of the European Social Fund. The system project of the Pomorskie Voivodeship "InnoDoktorant - Scholarships for PhD students, II edition".

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Authors (5)

Mariusz Jaszczołt mgr inż.
Anita Skrzypczak
Aleksandra Królicka
Lewandowski Aleksander
Marian Antoni Kamiński prof. dr hab. inż.

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: PLANTA MEDICA no. 77,
ISSN: 0032-0943
Language:: English
Publication year:: 2011
Bibliographic description:: Jaszczołt M., Skrzypczak A., Królicka A., Aleksander L., Kamiński M.: Optimal conditions of naphthoquinones separation from carnivorous plants extracts using thin-layer chromatography and high performance liquid chromatography// PLANTA MEDICA. -Vol. 77, nr. iss. 12 (2011),
DOI:: Digital Object Identifier (open in new tab) 10.1055/s-0031-1282249
Verified by:: Gdańsk University of Technology