Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory

Anna Brillowska-Dabrowska; Sanne Sogaard Nielsen; Henrik Vedel Nielsen; Maiken Cavling Arendrup

doi:10.3109/13693780903531579

Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory

Abstract

We recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%) yielded fungi in culture while the suspected etiologic agents were noted microscopically in 15 (13.8%) that were negative in culture. Fungi were detected by PCR in 37 (33.9%) samples, of which only three were positive in culture. Since the majority of PCR positive but culture negative samples were positive in microscopic examinations, the increased sensitivity was not due to contamination. PCR inhibitors were present in 5% of the samples, but this was overcome by re-running the samples with a 50% reduction of sample DNA. In conclusion, the PCR test performance in the routine setting was excellent and provided a markedly reduced time to diagnosis with a higher sensitivity.

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Publication version: Accepted or Published Version
DOI:: Digital Object Identifier (open in new tab) 10.3109/13693780903531579
License: Copyright (2010 ISHAM)

Keywords

DERMATOFITY

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: MEDICAL MYCOLOGY no. 48, pages 828 - 831,
ISSN: 1369-3786
Language:: English
Publication year:: 2010
Bibliographic description:: Brillowska-Dabrowska A., Nielsen S., Nielsen H., Arendrup M.: Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory// MEDICAL MYCOLOGY. -Vol. 48, nr. nr 6 (2010), s.828-831
DOI:: Digital Object Identifier (open in new tab) 10.3109/13693780903531579
Verified by:: Gdańsk University of Technology