Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status

Joanna Golus; Magdalena Stankevič; Rafal Sawicki; Renata Los; Anna Malm; Grazyna Ginalska

doi:10.5301/ijao.5000157

Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status

Abstract

OBJECTIVES: This study aims to examine biofilm formed on vascular prostheses by Staphylococcus epidermidis with different ica and aap genetic status, and to evaluate the effect of antibiotic-modified prostheses on bacterial colonization. METHODS: Biofilm formation was determined using fluorescence microscopy imaging. Quantitative analysis was conducted using the biofilm coverage ratio (BCR) calculations. RESULTS: Our investigations prove that the BCR method with fluorescent dye enabled an accurate assessment of biofilm coverage and comparison of the obtained results. The ica+ aap+ strains formed a biofilm on all of the examined vascular prostheses. Uni-Graft(®) modified with covalently immobilized amikacin was effective in preventing bacterial adherence. CONCLUSIONS: Molecular biology techniques combined with phenotype studies give a broad insight into biofilm formation mechanisms. On the other hand, fluorescence microscopy imaging along with BCR calculations are reliable and simple tools to quantitatively estimate biofilm formation, as well as the effectiveness of antimicrobial prosthesis modification.

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Authors (6)

Joanna Golus
Magdalena Stankevic (formerly: Magdalena Stankevic) dr inż.
- Uniwersytet Medyczny Lublin
Rafal Sawicki
Renata Los
Anna Malm
Grazyna Ginalska

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Keywords

STAPHYLOCOCCUS EPIDERMIDIS, BIOFILM, IMPLANT INFECTIONS, VASCULAR PROSTHESES, BIOMATERIAL SURFACE MODIFICATION

Details

Category:: Articles
Type:: artykuły w czasopismach
Published in:: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS no. 36, pages 105 - 112,
ISSN: 0391-3988
Language:: English
Publication year:: 2013
Bibliographic description:: Golus J., Stankevic M., Sawicki R., Los R., Malm A., Ginalska G.: Quantitative Analysis of Biofilm Formed on Vascular Prostheses by Staphylococcus Epidermidis with Different ica and aap Genetic Status// INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS -Vol. 36,iss. 2 (2013), s.105-112
DOI:: Digital Object Identifier (open in new tab) 10.5301/ijao.5000157
Verified by:: Gdańsk University of Technology