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Rapid determination of multiple aminoglycoside antibiotics in veterinary formulations by ion-pair chromatography coupled with evaporative light scattering detection
Abstract
A fast and simple method for simultaneous determination of eight aminoglycoside antibiotics using ion-pairing liquid chromatography (IPLC) coupled with evaporative light scattering detection (ELSD) has been developed and validated. Separation of amikacin, apramycin, gentamicin (as a sum of gentamicin C1, C2 and C1A), kanamycin A, neomycin B, paromomycin, streptomycin and tobramycin was achieved using C18 column with H2O and MeOH/acetone, both with addition of nonafluoropentanoic acid. Limits of detection ranged between 1.2 μg/mL and 2.8 μg/mL. The total analysis time was 9.5 min. The developed method showed the suitability for the routine quality control of veterinary drugs (drops, powders and paraffin-based suspensions).
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.microc.2021.106843
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- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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MICROCHEMICAL JOURNAL
no. 171,
ISSN: 0026-265X - Language:
- English
- Publication year:
- 2021
- Bibliographic description:
- Glinka M., Wasik A.: Rapid determination of multiple aminoglycoside antibiotics in veterinary formulations by ion-pair chromatography coupled with evaporative light scattering detection// MICROCHEMICAL JOURNAL -Vol. 171, (2021), s.106843-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.microc.2021.106843
- Verified by:
- Gdańsk University of Technology
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